葛根素对噪声慢性应激大鼠行为学及脑内记忆相关蛋白表达的影响

Effects of Puerarin on the Behavior and Expression of Memory-related Proteins in Chronic Noise Stressed Rats

目的探讨葛根素对噪声刺激下慢性应激大鼠行为学及脑内记忆相关蛋白的影响。方法生长期SD雄性大鼠32只,随机分为正常对照组、模型对照组和葛根素小、大剂量(100,200 mg·kg^(-1))组,每组8只。通过噪声连续刺激60 d建立慢性应激大鼠模型。采用糖水消耗实验、水迷宫实验观察大鼠行为学变化;使用免疫印迹法(Western blotting)和酶联免疫吸附测定(ELISA)法检测脑海马组织的记忆相关蛋白香草酸受体亚型1(TRPV1)、蛋白激酶C(PKC)、磷酸化环磷腺苷效应元件结合蛋白(p-CREB)、脑源性神经营养因子(BDNF)表达变化。结果与正常对照组比较,模型对照组和葛根素给药组大鼠体质量增加较缓慢,糖水消耗量下降;水迷宫实验中,与正常对照组比较,模型对照组逃避潜伏期明显延长,在目标象限游泳总路程明显下降,TRPV1、PKC表达显著上调,p-CREB、BDNF表达显著下调(P<0.05)。葛根素小剂量组大鼠逃避潜伏期明显缩短,在目标象限游泳总路程有所上升,TRPV1、PKC表达显著下调,p-CREB、BDNF表达显著上调(P<0.05)。结论长期噪声应激影响生长期大鼠的学习记忆能力,葛根素可部分改善慢性应激大鼠的学习记忆能力,其作用机制可能与调节脑海马记忆相关蛋白表达水平有关。

Objective To investigate the effects of puerarin on behavior and memory-related proteins in rats with chronic stress induced by noise.Methods Thirty-two SD male rats were divided into normal control group,model control group,low dose(100 mg·kg^(-1))puerarin group and high dose(200 mg·kg^(-1))puerarin group(n=8 in each group).The rat model of chronic stress was established by continuous noise stimulation for 60 days.Sugar consumption test and water maze test were carried out.The expression of memory-related proteins,such as transient receptor potential vanilloid subfamily member 1(TRPV1),protein kinase C(PKC),phosphorylated cAMP-response element binding protein(p-CREB)and brain derived neurotrophic factor(BDNF),in hippocampus were detected by Western blotting and ELISA.Results Compared with normal control group,the body mass of rats in the model control group and the puerarin group increased slower,and the consumption of sugar water decreased.In the water maze test,the escape latency of model control group was significantly longer than that of the normal control group,and the total swimming distance in the target quadrant was significantly decreased.The expression of TRPV1 and PKC was significantly up-regulated,and the expression of p-CREB and BDNF was significantly down-regulated.There was statistical significance(P<0.05).After treatment with 100 mg·kg^(-1)puerarin,the escape latency was significantly shortened,the total swimming distance in the target quadrant was increased,the expression of TRPV1 and PKC was significantly down-regulated,and the expression of p-CREB and BDNF was significantly up-regulated in the low dose puerarin group.There was statistical significance(P<0.05).Conclusion Long-term noise stress affects the learning and memory ability of the growing rats,whereas puerarin can partially improve the learning and memory ability of chronic stress rats,and its mechanism may be related to adjust the expression level of memory-related proteins in hippocampus.