葵花盘霉变标志物的制备及在中药安全性评价中的应用

Preparation of Mildew Markers of Helianthi Annui Receptaculum and Its Application in the Safety Evaluation of Traditional Chinese Medicine

目的制备葵花盘霉变标志物,并探讨其在中药安全性评价中的应用。方法采用柱色谱、薄层色谱等技术,分离制备葵花盘霉变标志物。建立以薄层色谱技术为基础的葵花盘霉变安全性评价方法,收集12种霉变药材对方法的适用性进行考察。结果葵花盘、槐米、陈皮、人参等霉变样品的薄层色谱与霉变标志物相比,均显相同荧光斑点。结论所建立的葵花盘安全性评价方法灵敏、专属性强,对多糖、皂苷及蛋白质含量较高的植物药材的霉变评价具有一定的适用性,但不适用于菌类药物。

Objective To find out a mildew marker of Helianthi annui receptaculum and to assess its application study in safety evaluation of traditional Chinese medicine.Methods The mildew marker of Helianthi annui receptaculum was isolated and prepared by column chromatography and thin layer chromatography(TLC).An evaluation method for Helianthi annui receptaculum was established based on TLC technology.Twelve kinds of mildew Chinese medicine were collected to evalute the applicability of the method.Results TLC of mildew samples,including Helianthi annui receptaculum,Flos Sophora,Pericapium Citri Reticulate and Panax ginseng showed the same fluorescence compared with mildew markers.Conclusion The established method for evaluating the coiledew of sunflower is sensitive and specific.It has certain applicability to the evaluation of mildew of plant medicinal materials with high content of polysaccharides,saponins and proteins,but it is not suitable for medicinal fungi.