摘要
目的:研究硫炔丙基半胱氨酸(S-propargyl-cysteine,SPRC)溶液对牙周炎常见致病菌的体外抗菌作用。方法:体外分别将牙龈卟啉单胞菌(P.gingivalis),中间普氏菌(P.intermedia)和粪肠球菌(E.faecalis)与不同浓度SPRC(10,20,50和100 mmol/L)共培养,没有给药处理的作为对照组(0 mmol/L组),给予2%醋酸氯己定(Chlorhexidine acetate,CHA)处理作阳性对照组;每间隔1 h测定1次各浓度组共培养后的吸光度(OD600nm)值,并绘制10 h生长曲线;采用直接接触法(direct contact test,DCT)和CCK-8法测定不同浓度下共培养5 h后各组吸光度值;采用菌落形成实验(colony-forming units,CFU)检测37℃缺氧条件培养24 h后不同浓度组对菌落数形成的差异。结果:SPRC对E.faecalis生长有影响,在5~10 h内不同浓度给药组与对照组(0 mmol/L组)相比细菌数量明显减少(P<0.05);DCT,CCK-8和CFU法结果均显示SPRC对E.faecalis具有抑菌作用,不同浓度给药组与对照组(0 mmol/L组)相比具有明显差异(P<0.05),且高浓度抑制作用更明显。DCT,CCK-8和CFU法结果显示不同浓度SPRC对P.gingivalis、P.intermedia无抗菌性,均无统计学差异(P>0.05)。结论:体外研究发现SPRC对E.faecalis具有抗菌性,且效果随浓度增加而增强,但对P.gingivalis和P.intermedia无抑制作用。
Objective:To investigate the antibacterial effect of S-propargyl-cysteine(SPRC)solution on common pathogenic bacteria of periodontitis in vitro.Methods:Different concentrations of SPRC(10,20,50,100 mmol/L)was co-cultured with Porphyromonas gingivalis(P.gingivalis),Porphyromonas intermedia(P.intermedia)and Enterococcus faecalis(E.faecalis)in vitro.Untreated group is served as control group(0 mmol/L)and 2%CHA treated group is served as the positive control group.The absorbance(OD600 nm)of each co-cultured concentration group was measured per hour and the total growth curve to 10 hours was analyzed.Direct contact method(DCT)and CCK-8 method were used to measure the absorbance of each group after co-cultivation in different concentrations for 5 hours.Colony formation experiment(CFU)was used to detect the difference in the number of formed colonies after 24 hours culture at 37°C under hypoxic conditions in different concentration groups.Results:SPRC significantly affects the growth of E.faecalis and the number of bacteria which was significantly lower in the SPRC groups(10,20,50,100 mmol/L)than the control group(0 mmol/L)from 5 to 10 hours(P<0.05).DCT,CCK-8 and CFU results showed that SPRC groups had bacteria-inhibiting effect to E.faecalis comparied with control group(0 mmol/L)(P<0.05)and the higher concentrations of SPRC groups would have the better antibacterial effect.However it showed no antibacterial effect on P.gingivalis and P.intermedia(P>0.05).Conclusion:SPRC has antibacterial activity against E.faecalis which was positively enhanced with the increased concentration,but has no inhibitory effect on P.gingivalis and P.intermedia in vitro.
作者
彭前
祁胜财
赵秉坤
从少华
汪饶饶
PENG Qian;QI Sheng-cai;ZHAO Bing-kun;CONG Shao-hua;WANG Rao-rao(Shanghai No.10 People’s Hospital Affiliated to Tongji University,Shanghai 200040,China;Jiading Central Hospital,Shanghai University of Medicine&Health Sciences,Shanghai,200011,China)
出处
《临床口腔医学杂志》
2021年第3期131-135,共5页
Journal of Clinical Stomatology
基金
上海市卫生和计划委员会科研课题(20184Y0110)。