近红外光谱法检测山茱萸中莫诺苷和马钱苷的含量

Determination of monoside and maganoside in Fructus corni by near infrared spectroscopy

目的建立近红外光谱法快速检测山茱萸中莫诺苷和马钱苷含量的分析方法。方法利用近红外光谱仪扫描粉碎后的山茱萸药材样品,对其光谱进行预处理和波段选择,并结合偏最小二乘法(partial least squares,PLS)建立莫诺苷和马钱苷含量快速无损检测方法。结果所建立的模型的决定系数R分别为0.9668、0.9062,交叉验证均方根差值分别为0.059、0.047,对验证集样品进行预测并统计分析,预测值与真实值之间无显著差异(P>0.05)。结论所建立的模型准确度高,适用于山茱萸药材粉末的莫诺苷和马钱苷含量的快速检测。

Objective To establish a method for the rapid determination of monoside and maganoside of Fructus corni by near infrared spectroscopy.Methods The near infrared spectroscopy was used to scan the the crushed samples of Fructus corni.The spectra of the samples were pretreated and the bands were selected.A fast and nondestructive method for the content determination of monoside and maganoside was established by using partial least squares(PLS)method.Results The determination coefficient R of the established model was 0.9668 and 0.9062,and the root-mean-square difference of cross-validation was 0.059 and 0.047,respectively.The validation set samples were predicted and statistically analyzed,and there was no significant difference between the predicted value and the true value(P>0.05).Conclusion The model has high accuracy and is suitable for the rapid determination of monoside and maganoside in the powder of Fructus corni.