参芪降糖颗粒提取工艺的优化及定性鉴别

Optimization of extraction process and qualitative identification of ginseng-milkvetch declining sugar granule

目的优化参芪降糖颗粒的提取工艺,初步建立其薄层色谱的鉴别方法。方法采用水煎煮法对参芪降糖颗粒进行提取,以干浸膏得率为评定指标,通过正交试验设计对煎煮时间、煎煮次数和加水量进行考察,优选参芪降糖颗粒的提取工艺,采用薄层色谱法(thin layer chromatography,TLC)对参芪降糖颗粒中的黄芪\葛根\丹参进行TLC定性鉴别。结果最佳提取工艺为处方药材加10倍水,提取2次,每次1.5 h。TLC图斑点清晰、重现性好、阴性无干扰。结论参芪降糖颗粒提取工艺合理、可行,薄层鉴别方法简单可靠,为其开发利用提供科学依据。

Objective To optimize the extraction process of ginseng-milkvetch declining sugar granule and to establish its thin layer chromatography(TLC)identification method.Methods Ginseng-milkvetch declining sugar granule was extracted by water decoction,and the yield of dry extract was used as the evaluation index.The decocting time,decocting times and the amount of water added were investigated by orthogonal design.The extraction process of ginseng-milkvetch declining sugar granule was optimized by orthogonal experimental design The Astragalus membranaceus,Pueraria lobata and Salvia miltiorrhiza in ginseng-milkvetch declining sugar granule were qualitatively identified by thin layer chromatography.Results The best extraction technology was prescription herbs with 10 times water,extractiong two times,1.5 h each time.The spots of thin layer chromatography were clear,reproducible and negative without interference.Conclusion The extraction process of ginseng-milkvetch declining sugar granule is reasonable and feasible,and the TLC identification method is simple and reliable,which provides a scientific basis for its development and utilization.