FGF22及其受体在庆阳黑山羊正常睾丸与隐睾中的表达比较

Comparison of the Expression of FGF22 and Its Receptor in Normal Testicles and Cryptorchidism of Qingyang Black Goat

试验旨在研究成纤维生长因子22(fibroblast growth factor 22,FGF22)及其受体2(fibroblast growth factor receptor 2,FGFR2)、硫酸乙酰肝素糖蛋白(heparan sulfate proteoglycans,HSPG)在庆阳黑山羊正常睾丸与隐睾中的分布与表达,探究其在山羊睾丸发育和隐睾形成中的作用。采用HE和特殊染色观察其组织学结构特征,进而以免疫组织化学及免疫荧光法结合形态计量学统计研究FGF22、FGFR2和HSPG在山羊正常睾丸及隐睾中的定位。结果表明,山羊隐睾较正常睾丸生精小管缩窄,腔内各级生精细胞排列紊乱,间质的胶原纤维和网状纤维增多,糖原类物质阳性反应较弱,FGF22在隐睾组织的Leydig、Sertoli细胞、管周肌样细胞及血管内皮细胞整体表达密度相较于正常睾丸显著减弱(P<0.05)。HSPG在正常睾丸表达显著强于隐睾(P<0.05),间质组织变化尤其明显。FGFR2在隐睾组表达显著增高(P<0.05),且以Sertoli细胞强阳性表达为主。庆阳黑山羊隐睾较正常睾丸发育异常,间质组织有纤维化趋势,糖原类物质含量减少;FGF22及HSPG表达降低应与隐睾局部环境温度变化密切相关;FGFR2在隐睾组表达增高提示其在发生隐睾时可能通过Sertoli细胞进行适应性调节。

This experiment was conducted to compare the distribution of the fibroblast growth factor 22(FGF22),fibroblast growth factor receptor 2(FGFR2),and the related regulatory factors heparan sulfate proteoglycans(HSPG)in normal testis and cryptorchidism of Qingyang Black goat,and explore their roles in testicular development and cryptorchidism.HE staining and special staining were used to observe the histological structure of FGF22,FGFR2 and HSPG,and the localization of FGF22,FGFR2 and HSPG in goat normal testis and cryptorchidism was studied by immunohistochemistry and immunofluorescence combined with morphometry.The results showed that the seminiferous tubules of cryptorchidism were narrower than those of normal testis,the arrangement of spermatogenic cells was disordered,the collagen fibers and reticular fibers in interstitium were increased,and the positive reaction of glycogen was weak.The overall expression density of FGF22 in Leydig,Sertoli cells,peritubular myoid cells and vascular endothelial cells of cryptorchidism was significantly lower than that of normal testis(P<0.05).The expression of HSPG in normal testis was significantly higher than that in cryptorchidism(P<0.05),especially in interstitial tissue.The expression of FGFR2 was significantly increased in cryptorchidism group(P<0.05),and Sertoli cells were the main positive cells.The results showed that the cryptorchidism of Qingyang Black goat was more abnormal than that of normal testis,the interstitial tissue had a tendency of fibrosis,and the content of glycogen decreased.The decreased expression of FGF22 and HSPG should be closely related to the changes of local environment temperature of cryptorchidism.The increased expression of FGFR2 in cryptorchidism group suggested that it might be regulated adaptively by Sertoli cells during cryptorchidism.