毛竹水溶性多糖提取工艺的优化及结构表征

Optimization of Polysaccharide Extraction from Bamboo Shoot and Its Characterization

采用响应面法优化毛竹水溶性多糖的提取工艺,分析其成分,并进行结构表征。研究结果表明,优化的毛竹多糖(BSP)提取条件为:料液比1∶27(g/mL)、提取温度78 ℃、提取时间4 h,该条件下BSP提取率为(5.18±0.07)%,提取物中多糖含量为(72.14±0.02)%,糖醛酸含量为(9.76±0.03)%;BSP具有酸性多糖典型的官能团特征吸收峰,其单糖组成及摩尔比为n_(甘露糖)∶n_(葡萄糖)∶n_(阿拉伯糖)∶n_(木糖)∶n_(鼠李糖)∶n_(半乳糖)=62.52∶23.05∶10.35∶2.78∶0.79∶0.51;BSP由两个主峰组成,相对分子质量分别为91.11、4.55 kDa。BSP是一种混合酸性多糖。

Response surface methodology was used to optimize the extraction process of water-soluble polysaccharides from bamboo shoots(Phyllostachys pubescensy),and the components and structure of the extracted polysaccharides were analyzed.The the optimum extraction conditions of bamboo polysaccharide(BSP)were as follows:solid-liquid ratio 27 g/mL,extraction temperature 78℃,extraction time 4 h,the yield of BSP was(5.18±0.07)%.The BSP contained(72.14±0.02)%polysaccharide and(9.76±0.03)%uronic acid.Infrared spectroscopy analysis showed that BSP had the characteristic absorption peaks of acidic polysaccharide.BSP was composed of mannose,glucose,arabinose,xylose,rhamnose,galactose in molar ratios of 62.52∶23.05∶10.35∶2.78∶0.79∶0.51.Gel chromatography showed that BSP was composed of two main peaks with a relative molecular mass of 91.11 and 4.55 kDa respectively,indicating that BSP is a mixed acidic polysaccharide.