牙痛停滴丸治疗口腔溃疡的药效学研究

Therapeutic effect of Yatongting Dropping Pills on oral ulcer in rats

目的研究牙痛停滴丸对大鼠口腔溃疡的治疗效果,并设计小鼠耳肿胀实验验证其抗炎作用。方法 SD大鼠随机分为对照组、模型组、牙痛停滴丸组(86.4 mg/kg,4倍临床等效剂量)、甲硝唑组(16.2 mg/kg,临床等效剂量,阳性药),每组10只。除对照组外,以80%冰醋酸溶液灼烧颊侧口腔黏膜的方法建立口腔溃疡模型,造模24 h开始给药,连续ig给药10 d,模型组和对照组给予等量蒸馏水。观察溃疡大体形态;于造模1 d和给药4、10 d,利用游标卡尺测量大鼠口腔溃疡的直径大小,进行口腔溃疡评分;于给药第4天,取每组2只大鼠溃疡处口腔黏膜做病理组织学观察。ICR小鼠随机分为模型组、阿司匹林(195 mg/kg,临床等效剂量,阳性药)组、牙痛停滴丸(124.8 mg/kg,4倍临床等效剂量)组,每天给药1次,连续ig 3 d。于末次给药1 h后,以二甲苯致小鼠耳肿胀为炎症模型,考察小鼠耳片的肿胀度。结果大体观察显示,给药10 d,模型组溃疡表面仍存在假膜,牙痛停滴丸组基本愈合,甲硝唑组溃疡面积减小并伴有假膜;与模型组比较,给药4、10 d时,牙痛停滴丸和甲硝唑组溃疡直径显著减小(P<0.05),口腔溃疡评分显著降低(P<0.05);HE染色显示,牙痛停滴丸组假膜较模型组小,且鳞状上皮细胞坏死程度较低。与模型组比较,牙痛停滴丸及阿司匹林组的小鼠耳片肿胀度均显著降低(P<0.05、0.01)。结论牙痛停滴丸可以治疗炎症、促进口腔溃疡的愈合,缓解溃疡表面的红肿情况,对口腔溃疡有一定的治疗作用。

Objective To study the therapeutic effect of Yatongting Dropping Pills(YTTDP) on oral ulcer in rats, and design mouse ear swelling experiment to verify its anti-inflammatory effect. Methods SD rats were randomly divided into control group, model group, YTTDP group(86.4 mg/kg, four times of clinical equivalent dose) and metronidazole group(16.2 mg/kg, clinical equivalent dose, positive drug), with 10 rats in each group. Expect for the control group, the buccal oral ulcer model was established by burning buccal mucosa with 80% glacial acetic acid solution. After 24 h of modeling, the drug was ig given continuously for 10 days. The model group and the control group were given the same amount of distilled water. The general morphology of ulcer was observed;the diameter of oral ulcer was measured by vernier caliper on the 1 st day of modeling and the 4 th and 10 th days of administration,and the score of oral ulcer was made;on the 4 th day of administration, the oral mucosa of two rats in each group was taken for histopathological observation. ICR mice were randomly divided into model group, aspirin(195 mg/kg, clinical equivalent dose,positive drug) group and YTTDP(124.8 mg/kg, four times clinical equivalent dose) group, the drug was ig given continuously for three dags. One hour after the last administration, xylene induced ear swelling in mice was used as the inflammatory model to investigate the swelling degree of mouse ear piece. Results Gross observation showed that there was still pseudomembrane on the ulcer surface in the model group after 10 days of administration, which was basically healed in the YTTDP group, and the ulcer area was reduced and accompanied with pseudomembrane in the metronidazole group. Compared with model group, the ulcer diameter and oral ulcer score of YTTDP and metronidazole groups were significantly decreased(P < 0.05). HE staining showed that the pseudomembrane of YTTDP group was smaller than that of model group, and the necrosis degree of squamous epithelial cells was lower. Compared with model group, the ear swelling degree of mice in YTTDP and aspirin groups were significantly decreased(P <0.05, 0.01). Conclusions YTTDP can treat inflammation, promote the healing of oral ulcers, relieve the redness on the surface of ulcers, and have a certain therapeutic effect on oral ulcers.