口腔鳞癌中FOXP3的表达及临床意义

Expression and clinical significance of FOXP3 in oral squamous cell carcinoma

目的探讨FOXP3在口腔鳞癌(OSCC)及癌旁组织中的表达及临床意义。方法采用免疫组化EnVision方法检测156例口腔鳞癌及96例癌旁组织芯片中FOXP3蛋白的表达水平,分析FOXP3表达与临床病理特征及预后的关系,并与TCGA数据库样本分析结果进行比较。结果 FOXP3在OSCC组织及癌旁组织中的高表达率分别为57.69%、20.83%,两者差异有统计学意义(P<0.000 1),与TCGA数据库分析结果一致,且FOXP3蛋白表达与T分期(P=0.003)、TNM分期(P=0.020)明显相关。Kaplan-Meier和COX回归分析显示,FOXP3高表达(P=0.012)、T分期(P=0.031)、TNM分期(P=0.032)是影响患者预后的相关因素。Cox多因素表明,FOXP3的高表达(P=0.009)是影响患者预后的独立因素。结论 FOXP3蛋白在OSCC组织中高表达,且与患者不良预后相关。

Objective To investigate the expression and clinical significance of FOXP3 in oral squamous cell carcinoma(OSCC)and adjacent tissues.Methods EnVision immunohistochemistry method was used to detect the expression level of FOXP3 protein in 156 cases of oral squamous cell carcinoma and 96 cases of adjacent tissue microarray.The relationships of FOXP3 expressions with clinicopathological characteristics and prognosis were analyzed,and the results were compared with TCGA database sample analysis results.Results The high expression rates of FOXP3 in the OSCC tissues and adjacent tissues were 57.69%and 20.83%,respectively and the difference the in expression rate was statistically significant between the groups(P<0.0001),which was consistent with the results from the TCGA database analysis.Its expression was significantly correlated with T staging(P=0.003)and TNM staging(P=0.020).By Kaplan-Meier and COX regression analysis,the related factors affecting the prognosis were high expression of FOXP3(P=0.012),T stage(P=0.031),and TNM stage(P=0.032).By COX multiple factors analysis,the independent factor affecting the prognosis was high expression of FOXP3(P=0.009).Conclusion The protein of FOXP3 is highly expressed in OSCC tissues and related to the poor prognosis of OSCC patients.