2019年辽宁省水痘-带状疱疹病毒基因特征分析

Genetic characterization of varicella-zoster virus in Liaoning province in 2019,China

目的分析2019年辽宁省收集到的水痘-带状疱疹病毒(VZV)基因特征。方法采集辽宁省2019年疑似水痘或带状疱疹患者的疱疹液样本共32份。采用实时荧光定量聚合酶链反应筛查疑似样本,通过检测分析阳性样本的三个开放阅读框(ORF)即ORF22、ORF38和ORF62上的单核苷酸多态性(SNP)位点,整理样本及序列资料并对基因序列对比分析,确定病毒株基因型别,进行疫苗株与野毒株鉴别。结果32份疑似水痘或带状疱疹患者样本中,27份为VZV阳性,均为野毒株属Clade2遗传支,核苷酸与氨基酸的同源性分别为98.85%~100%和96.9%~100%。其中LN-14在37990位点发生A→G碱基突变,并且有1例疫苗突破病例。结论2019年辽宁省流行的VZV基因型均属Clade2野毒株。

Objective To analyze the genetic characteristics of varicella-zoster virus(VZV)in Liaoning province in 2019.Methods Vesicle fluid samples were collected from 32 individuals with suspected VZV infection in Liaoning province from 2019.Nucleic acids were extracted,the suspected samples were screened by real-time fluorescent quantitative PCR.Single nucleotide polymorphisms(SNP)sites on ORF22,ORF38 and ORF62 were detected and analyzed in three open reading frames(ORFS)of positive samples.The samples and sequence data were collected and the gene sequences were compared to determine the genotype of the virus strain,and the vaccine strain was identified from the wild strain.Results In the 32 suspected samples,VZV-positive strains were detected in 27 samples,all belonged to wild-type strain of Clade 2.Compared with Clade 2 referenced strains,the homology of nucleotide and amino acid for all 10 samples were 98.85%-100%and 96.9%-100%,respectively.There was a synonymous mutation(A→G)in position 37990 of LN-14 strain.The nucleotide homology of ORF22 showed that all 27 samples were on the same branch with the Clade 2 referenced strains and had a vaccine breakthrough cases.Conclusions This study reveals that the VZV strains circulating in Liaoning province in 2019 were all wild-type strains belonging to Clade 2.