场效应放大样品堆积行为仿真

Simulation on field-amplified sample stacking behavior

为研究场效应放大的样品堆叠存在不均匀的电渗流,造成分析物富集受分子扩散和对流扩散影响的理论机制,在自主构建的场效应放大的样品堆叠物理模型基础上,基于COMSOL Multiphysics软件耦合了电势场、电导率场、流体场、分析物浓度场,提出了跨数量级疏密空间与时间定标分析的仿真计算策略。仿真结果表明:可通过优化微纳通道长度与富集时间,来控制分子扩散和对流扩散的影响,并获得了较好的分析物富集结果,富集倍比与电压值呈正相关。该研究结果可以为在微纳通道中富集带电分析物提供理论基础。

Nonuniform electroosmotic flow(EOF)in field-amplified sample stacking(FASS)causes analyte enrichment to be affected by molecular diffusion and convective diffusion.To study the theoretical mechanism behind,we propose a simulation calculation strategy of cross order density space and time calibration analysis on the basis of the self-constructed FASS physical model and COMSOL Multiphysics software,coupling the potential field,conductivity field,fluid field,and analyte concentration field.The simulation results show that the influence of molecular diffusion and convection diffusion can be controlled by optimizing the length of micro/nano-channel and enrichment time.Good enrichment results of analytes are obtained.The enrichment ratio is positively correlated with the voltage value.These results can provide a theoretical basis for the enrichment of charged analytes in micro/nano-channels.