摘要
目的研究陈皮酵素在体内抗肝纤维化的作用。方法119只雄性C57BL/6小鼠随机分为4组:模型组(n=49)、实验组(n=56)、对照组(n=7)和陈皮酵素组(n=7)。模型组灌胃40%CCl4橄榄油溶液(3×0.1 mL/周);实验组灌胃40%CCl4橄榄油溶液(3×0.1 mL/周)和陈皮酵素(0.26 mL/d);对照组和陈皮酵素组分别给予橄榄油(3×0.1 mL/周)和陈皮酵素(0.26 mL/d),共8周。采用腹腔注射CCl4的方法来构建小鼠肝纤维化模型,模型组和实验组每周处死7只小鼠,对照组和陈皮酵素组小鼠第8周处死,收集肝脏标本和血清。用HE染色和天狼星红染色观察各组小鼠肝组织病理改变,测定血清甘氨酸、丙氨酸氨基转移酶、谷氨酰胺转氨酶、碱性磷酸酶水平。结果HE和天狼星染色结果显示,只灌胃CCl4的模型组,肝纤维化程度逐渐加深,表现为汇管区明显的脂肪变性、坏死和炎性细胞浸润。但给予陈皮酵素的实验组炎症程度较模型组减轻,表现为空泡化和炎症细胞浸润减少,胶原沉积减少且实验组Ishak评分显著低于模型组(P<0.05)。与模型组相比,实验组血清肝功能指标甘氨酸、丙氨酸氨基转移酶、谷氨酰胺转氨酶、碱性磷酸酶水平显著降低(P<0.05)。结论陈皮酵素有肝保护作用,可以显著减轻肝纤维化程度。
Objective To investigate the inhibitory effect of Xinhui citrus fermentation liquor on liver fibrosis in mice.Methods Mouse models of liver fibrosis were established by intraperitoneal injection of CCl4 in 105 male C57BL/6 mice,followed by gavage of 0.1 mL 40%CCl4 olive oil 3 times a week(model group,n=49)or daily gavage of citrus liquor at the dose of 0.26 mL(citrus liquor group,n=56)for 8 weeks.Seven mice receiving only olive oil treatment(0.1 mL,3 times a week)and another 7 treated with citrus liquor served as the control group.Liver tissues and serum samples were collected from 7 mice in the citrus liquor group and model group each week and from the mice in the two control groups at the 8th week for pathological examination of the liver tissues using HE staining and Sirius red staining and for determination of the biochemical indexes of liver function.Results The mice in the model group showed progressively worsened liver fibrosis with obvious hepatic steatosis,necrosis and inflammatory cell infiltration.These liver pathologies were much ameliorated in citrus liquor group,which showed significantly reduced vacuolation,inflammatory cell infiltration,collagen deposition and the Ishak score of the liver tissue(P<0.05).Serum levels of cholyglycine,alanine aminotransferase,transglutaminase and alanine aminotransferase were all significantly lower in citrus liquor group than in the model group(P<0.05).Conclusion Xinhui citrus fermentation liquor has protective effect on the liver and can significantly ameliorate liver fibrosis in mice.
作者
曾兵
廖柏鸿
周东耀
白英明
陈恒星
陈柏忠
朱志博
ZENG Bing;LIAO Bohong;ZHOU Dongyao;BAI Yingming;CHEN Hengxing;CHEN Baizhong;ZHU Zhibo(Clinical Research Center,TCM-Integrated Hospital of Southern Medical University,Guangzhou 510315,China;Department of Clinical Laboratory,TCM-Integrated Hospital of Southern Medical University,Guangzhou 510315,China;Guangdong Xinbaotang Biological Technology Co,Ltd.,Jiangmen 529100,China)
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2021年第4期588-592,共5页
Journal of Southern Medical University
基金
广东省基础与应用基础研究基金(2020A1515010412)。
关键词
陈皮酵素
H&E染色
天狼星红染色
肝功能指标
抗肝纤维化
Xinhui citrus fermentation liquor
HE staining
Sirius red staining
liver function parameters
anti-liver fibrosis