长链非编码RNA AGAP2-AS1在胶质瘤增殖过程中作用机制的研究

Study on the Mechanism of Long Chain Non-coding RNA AGAP2-AS1 in Proliferation of Glioma

目的探究长链非编码RNA AGAP2-AS1在胶质瘤增殖过程中的作用机制。方法选取2018年12月-2019年11月期间的30例胶质瘤患者为观察组,同时期的30例脑外伤患者为对照组。比较两组的组织AGAP2-AS1 mRNA表达量、Ras及ERK1/2蛋白表达情况,并比较观察组中不同分级胶质瘤的AGAP2-AS1 mRNA表达量、Ras及ERK1/2蛋白表达情况,比较转染siRNA AGAP2-AS1与siRNANC的U87及U251细胞克隆数。结果观察组的RNA AGAP2-AS1 mRNA表达量、Ras及ERK1/2蛋白表达显著高于对照组,不同分级胶质瘤的RNA AGAP2-AS1 mRNA表达量、Ras及ERK1/2蛋白表达比较,差异有统计学意义(P<0.05),转染siRNA AGAP2-AS1的U87及U251细胞克隆数显著低于siRNA-NC,差异有统计学意义(P<0.05)。结论长链非编码RNA AGAP2-AS1可通过调控ERK/MAPK信号通路影响胶质瘤的增殖,下调AGAP2-AS1可显著影响胶质瘤细胞的克隆形成能力。

Objective To investigate the mechanism of long chain noncoding RNA AGAP2-AS1 in proliferation of glioma.Methods The specimens of 30 patients with glioma from December 2018 to November 2019 were chosen as observation group,specimens of 30 patients with traumatic brain injury at the same time were chosen as the control group.Then the tissue RNA AGAP2-AS1 mRNA expression,Ras and ERK1/2 protein expression of two groups were compared,then the tissue RNA AGAP2-AS1 mRNA expression,Ras and ERK1/2 protein expression of observation group with different classifications were compared,and the U87 and U251 cell clones of transfection siRNA AGAP2-AS1 and siRNANC were compared too.Results The RNA AGAP2-AS1 mRNA expression,Ras and ERK1/2 protein expression of observation group were significantly higher than those of control group,the Ras and ERK1/2 protein expression of observation group with different classifications were compared,there were statistically significant differences(P<0.05),the U87 and U251 cell clones of transfection siRNA AGAP2-AS1 were significantly lower than those of siRNA-NC,there were statistically significant differences(P<0.05).Conclusion The long chain non-coding RNA AGAP2-AS1 affect the proliferation of glioma by regulating ERK/MAPK signal pathway,and down regulation of AGAP2-AS1 can significantly affect the clonogenesis of glioma cells.