海南地区妊娠期高血压疾病患者母胎LMP和HLA-DM基因共享率研究

Association on the sharing rate of maternal fetal LMP and HLA-DM genes in women with pregnancy-induced hypertension in Hainan

目的探讨海南地区母胎LMP和HLA-DM基因共享率与妊娠期高血压疾病发病的关系。方法选择2016年8月—2018年8月在本院产科住院分娩的妊娠期高血压疾病母胎共60例,另外选择在同期本院分娩的正常母胎200对作为对照组。所有研究对象进行血样采集,提取外周血白细胞DNA。然后PCR扩增后进行基因测序。比较妊娠期高血压疾病患者和正常产妇母胎基因LMP和HLA-DM共享率的差异。结果当HLA-DM为2个共享时,妊娠期高血压疾病组比例明显高于正常组,具有显著性差异(P<0.01)。当LMP为4个共享时,妊娠期高血压疾病比例明显高于正常组,具有显著性差异(P<0.01)。妊娠期高血压疾病组患者HLA-DMB*0103位点、LMP2-R/H基因共享率最高,与对照组比较具有显著性差异(P<0.05)。其余位点共享率没有显著性差异(P>0.05)。结论海南地区妊娠期高血压疾病发病可能与母胎HLA-DM基因共享和LMP基因共享率有关,其中HLA-DMB*0103、LMP2-R/H可能是妊娠期高血压疾病的易感基因。

Objective To investigate the relationship between maternal-fetal LMP and HLA-DM gene sharing rate and the incidence of pregnancy-induced hypertension in Hainan.Methods A total of 60 mothers and fetuses with pregnancy-induced hypertension who gave birth in the obstetrics department of our hospital from August 2016 to August 2018 were selected.200 pairs of healthy pregnant women and their fetuses who gave birth in our hospital during the same period were selected as the control group.Blood samples were collected from all subjects,and DNA from peripheral blood leukocytes was extracted.Then PCR amplification followed by gene sequencing.To compare the difference in the sharing rate of LMP and HLA-DM between cases with pregnancy-induced hypertension and healthy controls.Results When HLA-DM was shared by two,the proportion of hypertensive disease group during pregnancy was significantly higher than that of the controls(P<0.01).When the LMP was shared by 4,the proportion of hypertension in pregnancy was significantly higher than that of the controlse(P<0.01).The HLA-DMB*0103 locus and LMP2-R/H gene sharing rate was the highest in the patients with hypertension in pregnancy,which was significantly different from the control group(P<0.05).There was no significant difference in the sharing rate of other sites(P>0.05).Conclusion The incidence of hypertension during pregnancy in Hainan may be related to sharing rate of LMP and HLA-DM genes among women with pregnancy-induced hypertension and their fetus in Hainan.Among them,HLA-DMB*0103 and LMP2-R/H may be susceptible genes for pregnancy-induced hypertension.