卵裂期胚胎可溶性人类白细胞抗原G与妊娠结局的关系

Association on Soluble Human Leukocyte Antigen G and Pregnancy Outcomes in Cleavage Embryos

目的探讨卵裂期胚胎培养液中可溶性人类白细胞抗原G(sHLA-G)表达与胚胎发育和妊娠结局的关系。方法选择2017年5月—2018年5月在江苏省苏北人民医院生殖医学中心接受冻融胚胎移植的86例不孕患者,共计100个周期为研究对象。根据妊娠结局分为妊娠组和未妊娠组,采取回顾性分析方法,比较两组基本信息及sHLA-G含量差异;再根据sHLA-G的测定结果分为sHLA-G阳性组及阴性组,比较两组的植入率。采用酶联免疫吸附实验(ELISA)检测卵裂期胚胎(体外培养第三天)培养液中sHLA-G浓度。结果妊娠组sHLA-G平均值高于非妊娠组,组间差异有统计学意义(P<0.05);sHLA-G阳性组的sHLA-G含量平均值及植入率均大于sHLA-G阴性组,差异有统计学意义(P<0.05);二元Logistic回归分析结果显示,胚胎培养液中sHLA-G含量为影响胚胎植入的独立危险因素(P<0.05)。结论第三天胚胎培养液中sHLA-G的表达及表达量与胚胎植入有关,可作为妊娠结局的预测指标。

Objective To investigate the relationship between the expression of soluble human leukocyte antigen G(sHLA-G)in cleavage medium of embryo and embryonic development and pregnancy obtained.Methods 86 patients of infertility who received Frozen-thawed embryo transfer at the Reproductive Medical Center of Northern Jiangsu People′s Hospital from May 2017 to May 2018 were selected,a total of 100 cycles were included in the study.According to the pregnancy outcomes,pregnant group and non-pregnant group were divided.The basic clinical data and difference of sHLA-G content between the two groups were compared by a retrospective analysis.Furthermore,the patients were divided into sHLA-G positive and negative group according to the result of sHLA-G measurement and the implantation rate was compared between the two groups.Enzyme-linked immunosorbent assay(ELISA)was used to detect the concentration of sHLA-G in the culture medium of cleavage.Results The average sHLA-G concentration was significantly higher in pregnant group than in non-pregnant group(P<0.05).The average sHLA-G concentration and the implantation rate of sHLA-G positive group were significant higher than sHLA-G negative group(P<0.05).The results of binary logistic regression analysis showed that the content of sHLA-G in embryonic culture medium was an independent risk factor for embryo implantation.Conclusion The expression of sHLA-G in D3 medium of embryo was associated with embryo implantation,which can be used as a potential marker of pregnancyoutcome.