摘要
为了探索Sr标记在鱼类耳石上留存的持久性,将1尾鲤(Cyprinus carpio)仔鱼在10 mg/L六水氯化锶(SrCl2·6H2O)溶液中浸泡标记2 d后,让其回到正常水体中恢复续养607 d(即20个月)时采样,再利用电子探针X射线微化学分析(EPMA)技术来确认其耳石上的Sr标记是否能长期存在。EPMA面分布和定量线分布结果均显示,即使在浸泡标记完成后640 d时,该鲤矢耳石、星耳石和微耳石上均能检测到明显的Sr标记区(但星耳石需要通过横截面来测定)。该标记方法在鲤增殖放流标记和效果评价中具有持久性。
In order to investigate the persistence of Sr markings on otoliths,the larvae common carp(Cyprinus carpio)were marked in this study,using a single concentration of high Sr solution for 2 d immersion.A recognizable Sr marking region could be detected in the otolith,after a long period of recovery,using electron probe X ray micro chemical analysis(EPMA)technique.The results of mapping analysis of Sr content and the fluctuation of Sr/Ca concentration ratios along line transect from the core(0μm)to the edge in otoliths showed that even after 607 d(i.e.,20 months)of recovery culture,a ring of red Sr marking region was still kept in the otolith.The Sr mark of otolith was firstly confirmed existence for quite a long time at least 20 months after exogenous Sr marking,suggesting that this method exactly have a strong stability,reliability,durability and application potential in the large-scale marking for fish proliferating and releasing and evaluation of corresponding effect.
作者
邱晨
姜涛
陈修报
刘洪波
杨健
QIU Chen;JIANG Tao;CHEN Xiu-bao;LIU Hong-bo;YANG Jian(Wuxi Fisheries College,Nanjing Agricultural University,Wuxi 214081,Jinagsu,China;Key Laboratory of Fishery Ecological Environment Assessment and Research Conservation in Middle and Lower Reaches of the Yangtze River,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,Jinagsu,China)
出处
《湖北农业科学》
2021年第8期114-117,共4页
Hubei Agricultural Sciences
基金
中央级公益性科研院所基本科研业务费专项(2019JBFM06号)
农业农村部农业财政专项(CJDC-2017-22号)。
