HEV经输血感染兔模型研究

Study on Rabbit Model of Hepatitis E Virus Infection by Blood Transfusion

目的建立输血感染戊型肝炎病毒(hepatitis E virus,HEV)兔模型,了解HEV全血输注传播的可能性,并观察感染后的指标动态。方法以兔HEV接种4只SPF兔,经腹腔静脉窦采集抗凝血,然后立即以约10 m L/kg经耳缘静脉注射输血方式分别给予正常SPF受体兔5只,每周监测受体兔粪便和血清抗原、抗体和核酸,连续13周。结果静脉输血后,5只受体兔均出现粪便排毒,其中3只受体兔引起慢性戊肝,排毒时间超过13周,同时出现病毒血症和HEV抗原阳性,HEV抗体阴性或弱阳性;2只受体兔引起急性戊肝,排毒时间均小于6周,同时HEV抗体检测Sample/Cut-off(S/CO)值均大于13、HEV抗原阴性、无病毒血症。结论通过输全血方式可以感染兔,并引起急性或慢性HEV感染。

Objective To establish a rabbit model of hepatitis E virus(HEV)infection via blood transfusion in order to investigate the possibility of HEV transmission through whole blood transfusion and observe the dynamics of HEV markers during infection.Method Anticoagulant-treated blood samples collected via posterior vena cava between the hepatic veins and diaphragm from four r HEV-infected specific pathogen free(SPF)rabbits,were transfused to five naive SPF rabbits(three of the blood samples were transfused to 3 recipient rabbits and the other sample to the 2 remaining recipient rabbits,respectively)through ear edge vein,with a dose of about 10 m L/kg.HEV RNA,antigen and anti-HEV antibody of the recipient rabbits were monitored weekly for 13 consecutive weeks.Result After intravenous transfusion,fecal HEV shedding occurred in all five rabbits;three of them had viremia and antigenemia.Anti-HEV antibody was negative or weakly positive;the other two rabbits were positive for anti-HEV antibody,but negative for antigen and viremia.The total success rate of the experimental infection was 5/5(100%).Among them,2/5(40%)rabbits had acute HEV infection,and 3/5(60%)rabbits showed chronic HEV infection as the infection persisted more than three months.Conclusion The rabbit model of HEV transfusion infection was successfully established.Acute and chronic HEV infection could occur in the infected rabbits.