响应面法优化亚临界萃取冰岛刺参内脏油脂工艺

Optimization on subcritical extraction of oil from Cucumariafrondosa visceral by response surface method

以富含脂类活性物质的冻干冰岛刺参内脏为原料,建立亚临界萃取冰岛刺参油脂新工艺。结果表明,亚临界萃取的最佳萃取工艺为料液比(m冰岛刺参∶V萃取液)1∶3(g/mL)、萃取压强0.4 MPa、萃取次数5次、萃取时间56.18min、萃取温度48.55℃,油脂萃取率为23.38%。通过对比不同方法萃取的油脂理化指标及气相色谱—质谱(GC-MS)图,亚临界法萃取的油脂理化指标均优于其他方法,油脂中EPA含量达40.7%,说明亚临界法萃取冰岛刺参油脂的工艺效率高,油脂品质更稳定。

A new process for subcritical extraction of Cucumariafrondosa oil was established using freeze-dried Cucumariafrondosa visceral which is rich in lipid active substances.The subcritical extraction conditions were optimized by single factor and response surface methodology,and the best extraction process was obtained:material-liquid ratio 1∶3(g/mL),extraction pressure 0.4 MPa,extraction times 5 times,extraction time 56.18 min,extraction temperature48.55℃,extraction rate 23.38%.By comparing the physical and chemical indexes of oil extracted by different methods and gas chromatography-mass spectrometry(GC-MS)graphs,the physicochemical indexes of oil extracted by subcritical method are better than other methods,the content of EPA in the oil reaches40.7%,indicating that the subcritical method for extracting Cucumariafrondosa oil has high process efficiency and more stable oil quality.