华东地区掌叶覆盆子的表型特征和基于ISSR标记的遗传分析

Phenotype Characteristics and Genetic Analysis Based on ISSR Makers of Rubus chingii in East China

目的:主产区掌叶覆盆子表型特征和遗传组成调查。方法:对华东地区24份掌叶覆盆子野生资源的表型进行观察并记录。基于简单重复间序列(ISSR)标记信息对24份掌叶覆盆子和5份悬钩子属近缘植物材料进行遗传分析。结果:24份掌叶覆盆子的表型变异丰富,通过茎干颜色、皮刺密度、叶形、幼叶色苷、花瓣长、果柄长、果型7个性状可以对华东地区主要的掌叶覆盆子资源进行区分;应用8条适于掌叶覆盆子研究的ISSR标记共检测到90个位点,其中多态性位点58个,占比64.44%;基于NTSYS-pc 2.10软件的相似性分析,ISSR标记可以明确地区分掌叶覆盆子与近缘悬钩子植物;在相似系数为0.67~0.70处,来自浙北、浙南和闽北3个区域的种质资源分别聚为1组;掌叶覆盆子物种内基因多样性指数(H)为0.2720、Shannon′s信息指数(I)为0.4127、遗传相似系数为0.5366~0.8780;3个区域群体间的遗传分化不明显,区域间群体的遗传距离<0.2538、遗传一致度>0.7759、基因分化系数(G st)为0.3203、基因流(N m)为1.0612。结论:华东地区掌叶覆盆子种质具有丰富的遗传多样性,可以应用ISSR标记对掌叶覆盆子进行遗传和系谱分析。

Objective:To investigate the phenotypic characteristics and genetic composition of Rubus chingii Hu in main producing area.Methods:The phenotype of 24 wild accessions of R.chingii in East China were observed and recorded.The genetic composition of 24 resources of R.chingii and the other 5 kinds of relative plant materials of Rubus were analyzed based on inter-simple sequence repeat(ISSR)marker data.Results:The phenotypes of 24 accessions of R.chingii were performed rich variation.Seven phenotypes including stalk color,stab density,leaf shape,anthocyanin of young leaf,petal length,fruit stalk length and fruit type,could be used to differentiate the main R.chingii accessions in East China.Eight ISSR markers were screened to research the genetic composition of R.chingii.Ampli cation from the accessions of the ISSR primers yielded 90 genetic loci,of which 58 loci was polymorphic,the percentage of polymorphic bands was 64.44%.Based on the similarity analysis by NTSYS-pc 2.10,R.chingii and the other relative plant materials of Rubus could be differentiated by ISSR makers;all of 24 accessions of R.chingii were divided into three subgroups,while the genetic similarity coef cient reached 0.67 to 0.70.The accessions from the region of Zhebei,Zhenan and Minbei clustered into one branch,respectively.Among accessions,the gene diversity index(H)was 0.2720;the Shannon′s information index(I)was 0.4127;the genetic identity were ranged 0.5366-0.8780.Among three subgroups,the genetic distance was less than 0.2538;the genetic identity was more than 0.7759;the genetic differentiation coefficient(G st)among subgroups was 0.3203;Gene flow(N m)was 1.0612,meaning that genetic differentiation among 3 regional populations was not obvious.Conclusion:The germplasm of R.chingii in East China had rich genetic diversity,and the genetic and pedigree analysis of R.chingii could be researched by using ISSR markers.