丰城鸡血藤的化学成分

Chemical constituents from the stem of Callerya nitida Benth.var.hirsutissima Z.Wei.

采用大孔吸附树脂、正、反相硅胶、Sephadex LH-20等多种柱色谱方法和制备高效液相色谱法对鸡血藤属植物丰城鸡血藤Callerya nitita Benth. var. hirsutissima. Z. Wei.藤茎70%乙醇提取物的石油醚和正丁醇萃取部位化学成分进行分离,并根据化合物的理化性质、波谱数据对化合物的结构进行鉴定。从两个萃取部位共分离鉴定了1个三萜类化合物和20个黄酮类化合物,即蒲公英赛酮(1)、槐角苷(2)、saikoisoflavonoside A(3)、鹰嘴豆芽素A 7-O-β-D-呋喃芹菜糖基-(1→5)-β-D-呋喃芹菜糖基(1→6)-β-D-吡喃葡萄糖苷(4)、刺芒柄花素-7-O-β-D-吡喃半乳糖苷(5)、3′-O-甲基红车轴草素(6)、鹰嘴豆芽素A 7-O-β-D-呋喃芹菜糖基-(1→2)-β-D-吡喃葡萄糖苷(7)、槐属双苷(8)、黄甘草苷(9)、4′-羟基-3′-甲氧基异黄酮-7-O-β-D-呋喃芹菜糖基-(1→6)-β-D-吡喃葡萄糖苷(10)、樱黄素(11)、樱黄素4′-O-β-D-吡喃葡萄糖苷(12)、红车轴草素-7-O-β-D-吡喃葡萄糖苷(13)、8-甲氧基异刺芒柄花素(14)、染料木素(15)、红车轴草素(16)、鹰嘴豆芽素A(17)、5,7-二羟基-3′,5′-二甲氧基异黄酮(18)、芒柄花苷(19)、异芒柄花素(20)、木犀草素(21)。其中化合物1~10、12~14、16~18、20为该植物中首次分离得到。

The chemical constituents from 70%ethanol petroleum ether and n-butanol extractions of Callerya nitita Benth.var.hirsutissima.Z.Wei.were separated by preparative high-performance liquid chromatographic techniques,including repeated column chromatography over macroporous adsorption resin,silica gel,ODS,Sephadex LH-20.The structures of the compounds were identified by their physicochemical properties,spectral data,and mass spectrometry data,in comparison with literature.In our research,one triterpenoids,taraxerone(1),and twenty flavonoids,including genistein-4′-O-β-glucoside(2),5-hydroxy-4′-methoxyisoflavone-7-O-β-Dglucopyranosyl-(1→6)-β-D-glucopyranoside(3),biochanin A 7-O-β-D-apiofuranosyl-(1→5)-β-D-apiofuranosyl-(1→6)-β-D-glucopyranoside(4),formononetin-7-O-β-D-galactopyranoside(5),5,7-dihydroxy-3′,4′-dimethoxyiso⁃flavone(6),biochanin A-7-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside(7),5,7-dihydroxyisoflavone-4′-O-α-L-rhamnopyranosyl-(1→2)-O-β-D-glucopyranoside(8),formononetin-7-O-D-apio-β-D-furanosyl(l→2)-β-D-glu⁃copyranoside(9),4′-hydroxy-3′-methoxyisoflavone-7-O-β-D-apiofuranosyl-(1→6)-β-D-glucopyranoside(10),prunetin(11),prunetin 4′-O-β-D-glucopyranoside(12),pratensein7-O-β-D-glucoside(13),8-methoxyisofor⁃mononetin(14),genistein(15),3′-hydroxybiochanin A(16),biochanin A(17),5,7-dihydroxy-3′,5′-dimethoxyiso⁃flavone(18),ononin(19),isoformononetin(20),5,7,3′,4′-tetrahydroxyflavone(21)were isolated from the two extract parts.Compounds 1-10,12-14,16-18,20 were obtained from this plant,and it is the first time to investi⁃gate the plant for the first time.