梨Trihelix转录因子家族成员鉴定及表达分析

Indentification and Expression Analysis of Trihelix Transcription Factor Family in Pear

Trihelix转录因子可以和光应答相关的GT元件结合,因此又称GT转录因子。本研究从梨基因组中鉴定出16个Trihelix家族基因,依次命名为PbGT1~PbGT16,从同属于蔷薇科的草莓和桃基因组中分别鉴定出11和16个Trihelix家族基因。染色体定位与基因复制事件分析表明,梨、草莓和桃Trihelix家族成员分别分布在12、6和8条染色体上,且梨Trihelix家族存在片段复制事件。种间系统进化树分析表明,梨、草莓和桃Trihelix家族成员分为6个亚族,梨家族成员(PbGT)属于GT-2、Subfamily O和SIP1亚族。结合进化关系及qRT-PCR验证,筛选出PbGT15可能参与调控梨果实石细胞团木质化。

Trihelix transcription factors(TFs)binded to light-responsive GT elements are called GT transcription factors. This study not only identified 16 members of the Trihelix family from the pear genome,named PbGT1–PbGT16 in turn,but also identified 11 and 16 members of the Trihelix family respectively from the genomes of strawberry and peach belonging to the Rosaceae family like pear. Chromosome localization showed that the Trihelix family members of pear,strawberry and peach were distributed on 12,6 and 8 chromosomes,respectively. Segmental duplication events were found in the Trihelix family of pear by gene duplication events analysis. The clustering result of interspecific phylogenetic tree showed that the Trihelix family members of pear,strawberry and peach were divided into six subfamilies and the Trihelix family members of pear(PbGTs) belong to the GT-2,Subfamily O and SIP1 subfamilies. Combined with evolutionary relationship and qRT-PCR verification,PbGT15 may be involved in regulating lignification of pear fruit stone cells.