清肺化痰颗粒对慢性阻塞性肺疾病急性加重期大鼠相关炎症因子及氧化应激反应的影响研究

Effects of Qingfei Huatan granules on related inflammatory factors and oxidative stress reaction in rats with acute exacerbation of chronic obstructive pulmonary disease

目的观察清肺化痰颗粒对慢性阻塞性肺疾病急性加重期(AECOPD)大鼠相关炎症因子及氧化应激反应的影响。方法将60只雄性Wistar大鼠随机分为正常组、模型组、琥乙红霉素组及清肺化痰颗粒高、中、低剂量组,每组10只。除正常组外,其余组进行AECOPD造模。造模成功后,琥乙红霉素组给予琥乙红霉素3.15 g/kg灌胃,清肺化痰颗粒高、中、低剂量组分别给予清肺化痰颗粒9.94 g/kg、4.97 g/kg、2.49 g/kg灌胃,正常组和模型组给予生理盐水灌胃,均1次/d,连续14 d。观察大鼠症状行为学和肺组织病理变化,检测血清和肺组织中白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)、基质金属蛋白酶-9(MMP-9)、组织金属蛋白酶抑制剂-1(TIMP-1)、丙二醛(MDA)、超氧化物歧化酶(SOD)水平。结果模型组大鼠肺组织病理半定量评分显著高于正常组(P<0.05);血清和肺组织中IL-6、TNF-α、IFN-γ、MMP-9、TIMP-1、MDA水平及MMP-9/TIMP-1均显著高于正常组(P均<0.05),SOD水平显著低于正常组(P<0.05)。琥乙红霉素组及清肺化痰颗粒高、中、低剂量组肺组织病理半定量评分均显著低于模型组(P均<0.05);血清和肺组织中IL-6、TNF-α、IFN-γ、MMP-9、TIMP-1、MDA水平及MMP-9/TIMP-1均显著低于模型组(P均<0.05),SOD水平显著高于模型组(P均<0.05)。清肺化痰颗粒高、中剂量组肺组织病理半定量评分显著低于琥乙红霉素组(P均<0.05);血清和肺组织中IL-6、TNF-α、IFN-γ、MMP-9、TIMP-1、MDA水平及MMP-9/TIMP-1均显著低于琥乙红霉素组(P均<0.05),SOD水平显著高于琥乙红霉素组(P均<0.05)。结论高、中剂量清肺化痰颗粒可以有效改善AECOPD大鼠的症状,减轻病理损伤,其机制可能与降低IL-6、TNF-α、IFN-γ、MMP-9/TIMP-1,纠正氧化应激失衡状态有关。

Objective It is to observe the effects of Qingfei Huatan granules on related inflammatory factors and oxidative stress in rats with acute exacerbation of chronic obstructive pulmonary disease(AECOPD).Methods Sixty male Wistar rats were randomly divided into normal group,model group,erythromycin ethylsuccinate group and Qingfei Huatan granule high,medium and low dose groups,each group had 10 rats.Except for the normal group,the other groups underwent AECOPD modeling.After successful modeling,the erythromycin ethylsuccinate group was given 3.15 g/kg erythromycin ethylsuccinate by gavage,and the high,medium and low dose groups of Qingfei Huatan granules were respectively given 9.94 g/kg,4.97 g/kg and 2.49 g/kg Qingfei Huatan granules bt gavage,the normal group and the model group were given normal saline by gavage,all once per day,all the groups were treated for 14 days.The changes of symptomatic behavior and pathological changes of lung tissue in the rats were observed,and the levels of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ),matrix metalloproteinase-9(MMP-9),tissue inhibitor of metalloproteinase-1(TIMP-1),malondialdehyde(MDA),superoxide dismutase(SOD)in serum and lung tissue were detected.Results The semi-quantitative score of lung tissue in the model group was significantly higher than that in the normal group(P<0.05);the levels of IL-6,TNF-α,IFN-γ,MMP-9,TIMP-1,MDA and MMP-9/TIMP-1 in serum and lung tissue were significantly higher than the normal group(P<0.05),and the SOD level was significantly lower than the normal group(P<0.05).The semi-quantitative scores of lung tissue in the erythromycin ethylsuccinate group and the high,medium and low dose groups of Qingfei Huatan granules were significantly lower than those of the model group(P<0.05);the levels of IL-6 and TNF-α,IFN-γ,MMP-9,TIMP-1,MDA and MMP-9/TIMP-1 in serum and lung tissues were significantly lower than those of the model group(all P<0.05),and the SOD level was significantly higher than that of the model group(all P<0.05).The semi-quantitative scores of lung tissue in the Qingfeihuatan granules high and medium dose groups were significantly lower than those of the erythromycin ethylsuccinate group(P<0.05);the levels of IL-6,TNF-α,IFN-γ,MMP-9,TIMP-1,MDA and MMP-9/TIMP-1 in serum and lung tissue were significantly lower than those of the erythromycin ethylsuccinate group(all P<0.05),and the SOD level was significantly higher than that of the erythromycin ethylsuccinate group(all P<0.05).Conclusion The high and medium dose Qingfei Huatan granules can effectively improve the symptoms of AECOPD rats and reduce the pathological damage.The mechanism may be related to the reduction of IL-6,TNF-α,IFN-γ,MMP-9/TIMP-1,and the correction of oxidation stress imbalance.