摘要
目的探讨黑大蒜多糖对X射线辐射损伤小鼠的防护作用.方法将75只雄性昆明小鼠按体质量随机分为空白对照组、辐射模型组、黑大蒜多糖低、中、高剂量组(给药剂量分别为150、300、600 mg/kg).小鼠于辐射前进行预防性给药,黑大蒜多糖各剂量组每日分别灌胃2 mL不同浓度黑蒜多糖提取物,空白对照和辐射模型组灌胃2 mL生理盐水,连续灌胃14 d后,除空白对照组外,使用直线加速器对各组动物进行全身X射线照射,源皮距为100 cm,照射野为20 cm×20 cm,辐射剂量率为400 cGy/min,每日辐射总吸收剂量为2 Gy,连续照射2 d.辐射后,分别检测小鼠胸腺和脾脏指数、血常规、肝脏组织中抗氧化相关酶活性,观察内源性脾结节数和骨髓嗜多染红细胞微核率.结果与空白对照组比较,辐射模型组小鼠胸腺和脾脏指数降低,CFU-S数和骨髓微核率增加,WBC和PLT计数降低,T-SOD、LDH、T-AOC活力和GSH含量下降.与辐射模型组比较,黑大蒜多糖600 mg/kg组可增高胸腺指数、脾脏指数、PLT计数及LDH、T-AOC活力;300 mg/kg组可提高WBC计数;150、300、600 mg/kg组可增加CFU-S数、T-SOD活力、GSH含量,降低骨髓微核率.结论黑大蒜多糖可减轻X射线辐射对小鼠免疫器官和全血WBC、PLT的影响,能够增强辐射后小鼠肝脏抗氧化能力,减轻辐射诱导的氧化损伤,降低骨髓微核率,具有较强的抗辐射损伤作用.
Objective To investigate the protective effect of black garlic polysaccharide on mice damaged by X-ray radiation.Method Seventy-five male Kunming mice were divided into control group,radiation model group and black garlic polysaccharide low,medium and high dose group(150,300 and 600 mg/kg).In the experimental group,2 mL of black garlic polysaccharide with different concentrations was gavaged daily.Control group and model group were gavaged with 2 mL normal saline.After continuous administration for 14 days,except the control group,mice were subjected to X-ray irradiation for 2 d,with a radiation dose rate of 400 cGy/min and a radiation dose of 2 Gy/d.The thymus index,spleen index,blood routine and antioxidant enzyme activity of liver tissues were measured,and the CFU-S count and bone marrow micronucleus rate were observed.Results Compared with the control group,the thymus index and spleen index of mice in the radiation model group decreased,CFU-S and bone marrow micronucleus rate increased,WBC and PLT decreased,and T-SOD,LDH,T-AOC and GSH decreased.Compared with the radiation model group,the thymus index,spleen index,PLT,LDH and T-AOC increased in the black garlic polysaccharide group(600 mg/kg),WBC increased in the group(300 mg/kg),CFU-S,T-SOD and GSH increased in the group(150,300 and 600 mg/kg),and the micronucleus rate of bone marrow decreased.Conclusion Black garlic polysaccharides could reduce the effects of X-ray radiation on immune organs,WBC and PLT in mice.It could reduce the oxidative damage caused by radiation and reduce the micronucleus rate of bone marrow.
作者
胡淼
徐鸿洁
常哲兴
HU Miao;XU Hongjie;CHANG Zhexing(Affiliated Hospital of Beihua University,Jilin 132011,China)
出处
《北华大学学报(自然科学版)》
CAS
2021年第2期202-206,共5页
Journal of Beihua University(Natural Science)
基金
吉林省教育厅科学技术研究项目(JJKH20180360KJ).
关键词
黑蒜多糖
X射线
辐射损伤
小鼠
辐射防护
black garlic polysaccharide
X-ray
radiation injury
mice
radioprotection