在猪肺泡巨噬细胞上Notch信号通路调控LPS诱导的炎性反应研究

Notch Signaling Regulates LPS-induced Inflammatory Responses in Porcine Alveolar Macrophages

Notch信号通路可以与TLR信号通路相互作用,协作调控炎性因子的产生以及巨噬细胞的激活。然而,在猪巨噬细胞上,Notch信号通路是如何调控炎性反应的还不清楚。本研究以LPS/TLR4诱导炎性反应为模型,利用猪肺泡巨噬细胞来研究LPS处理对Notch信号通路的影响及Notch信号通路对LPS诱导炎性反应的调控作用。结果显示:LPS处理增加配体Jagged1、Jagged2和Dll4的表达,并上调下游靶基因Hey1的表达;Notch信号通路调控LPS诱导的炎性反应。本研究结果不仅明确了在猪巨噬细胞上Notch信号通路调控LPS诱导的炎性反应,而且为将来揭示Notch信号通路在猪相关细菌感染中的作用奠定基础。

The Notch signaling and TLR signaling can interact cooperatively with each other to regulate the production of inflammatory factors and activate the macrophages.However,it is little known about how the Notch signaling regulates the inflammatory responses in porcine alveolar macrophages (PAMs).In this study,a model study was established in PAMs for inflammatory responses induced by LPS/TLR4 in order to investigate effect of LPS treatment on Notch signaling and regulation of LPS-induced inflammatory responses by Notch signaling.The results showed that LPS treatment increased the expression of ligands Jagged1,Jagged2 and Dll4,and up-regulated the expression of downstream target gene Hey1.Furthermore,the Notch signaling regulated LPS-induced inflammatory responses as demonstrated using DAPT treatment and silence RBP-J expression.These findings revealed that the Notch signaling in PAM regulated the LPS-induced inflammatory responses and paved the way for investigation of the Notch signaling in pig-associated bacterial infections.