高温胁迫下尼罗罗非鱼肝脏组织的转录组分析

Transcriptome analysis of liver tissue of Nile tilapia Oreochromis niloticus exposed to high temperature stress

为探究罗非鱼高温胁迫响应的调控作用机理,选用孵化12 d后的尼罗罗非鱼Oreochromis niloticus幼鱼,基于RNA-seq技术对尼罗罗非鱼高温处理组(36℃高温胁迫下养殖30、50、70 d)和常温对照组(28℃,30、50、70 d)肝脏组织样品进行转录组分析。结果表明:高温处理组与常温对照组共获得39.23 Gb clean data;28℃-30 d与36℃-30 d文库比较发现,存在4342个差异基因,28℃-50 d与36℃-50 d文库比较发现,存在3139个差异基因,28℃-70 d与36℃-70 d文库比较发现,存在3042个差异基因;进一步将差异表达基因进行GO功能注释和KEGG富集分析,发现差异基因主要富集在糖酵解/糖异生、细胞周期、内质网的蛋白质加工及胰岛素信号等通路上;通过RT-qPCR试验对mTOR信号通路及相关通路中的8个差异基因进行验证,证实了转录组测序结果的可靠性。研究表明,高温胁迫下尼罗罗非鱼肝脏组织中参与热应激相关的基因涉及生长、蛋白质折叠及能量代谢等多个生物学过程,本研究结果为深入研究罗非鱼高温胁迫响应调控机制奠定了基础。

In order to explore the response mechanism of tilapia Oreochromis to high temperature stress,transcriptome analysis was conducted in liver tissue samples collected from Nile tilapia Oreochromis niloticus cultured for 12 days exposed to 36℃(high-temperature group)and to 28℃(control group)for 30,50,and 70 d by RNA-seq technology.The results showed that there was a total of 39.23 Gb of clean data in Nile tilapia in the high-temperature group and the normal temperature control group.Comparison revealed that there were 4342 differentially expressed genes in libraries 28℃-30 d and 36℃-30 d;3139 differentially expressed genes in libraries 28℃-50 d and 36℃-50 d;and 3042 differentially expressed genes in libraries 28℃-70 d and 36℃-70 d.Further,GO and KEGG analysis indicated that high temperature stress was involved in multiple biological pathways,mainly glycolysis/gluconeogenesis,cell cycle,endoplasmic reticulum protein processing signaling pathway,mTOR signaling pathway and insulin signaling pathway.Finally,8 differential genes were verified in the mTOR signaling pathway and related pathways by RT-qPCR experiments,which confirmed the reliability of the transcriptome sequencing results.The findings indicated that genes involved in high temperature stress in the liver tissue of Nile tilapia were involved in multiple biological processes including growth,protein folding and energy metabolism,and that provided the foundation for in-depth study of the regulation mechanism of tilapia in response to high temperature stress.