慢性收缩性心力衰竭患者血清miR-129表达与心功能的关系

The relationship between serum miR-129 expression and cardiac function in patients with chronic systolic heart failure

目的探讨慢性收缩性心力衰竭(CSHF)患者血清微小RNA-129(miR-129)表达水平并分析其与心功能指标的关系。方法选取2016年5月—2018年10月在武汉市第六医院收治的103例CSHF患者为观察组,依据纽约心脏病协会(NYHA)标准将其分为三组:NYHAⅡ级组(33例)、NYHAⅢ级(28例)、NYHAⅣ级组(42例)。另外选取110例同期健康体检者为对照组。采用飞利浦iEElite型心脏彩色多普勒超声仪检测左心室收缩期末内径(LVESD)、左心室舒张期末内径(LVEDD)、左心室射血分数(LVEF),采用实时荧光定量PCR(qRT-PCR)检测血清miR-129表达,并分析miR-129与LVESD、LVEDD、LVEF、NYHA分级的相关性。结果在对照组、NYHAⅡ级组、NYHAⅢ级组、NYHAⅣ级组中,LVESD、LVEDD均依次升高(P<0.05),LVEF和血清miR-129表达均依次降低(P<0.05);血清miR-129表达水平在冠心病、高血压心脏病、老年性心瓣膜病CSHF患者中差异无统计学意义(P>0.05);CSHF患者血清miR-129表达与LVESD、LVEDD、NYHA分级均呈负相关(P<0.05),与LVEF呈正相关(P<0.05)。结论miR-129在CSHF患者血清中低表达,与LVESD、LVEDD、NYHA分级均呈负相关,与LVEF呈正相关,可为CSHF的病情评估提供参考。

Aim To investigate the expression of microRNA-129(miR-129)in serum of patients with chronic systolic heart failure(CSHF)and analyze its relationship with cardiac function indicators.Methods 103 patients with CSHF who were treated in Wuhan Sixth Hospital from May 2016 to October 2018 were selected as the observation group.According to the New York Heart Disease Assocation(NYHA)standard,the observation group was divided into three groups:NYHA classⅡgroup(33 cases),NYHA classⅢgroup(28 cases),NYHA classⅣgroup(42 cases).In addition,110 healthy people from Wuhan Sixth Hospital at the same time were selected as the control group.The left ventricular end systolic diameter(LVESD),left ventricular end diastolic diameter(LVEDD),left ventricular ejection fraction(LVEF)were measured by Philips iEElite type echocardiography,real-time fluorescence quantitative PCR(qRT-PCR)was used to detect the expression of miR-129 in serum,and the correlation between miR-129 and LVESD,LVEDD,LVEF and NYHA classification was analyzed.Results Compared with the control group,the LVESD and LVEDD increased significantly in NYHA classⅡgroup,NYHA classⅢgroup and NYHA classⅣgroup(P<0.05),while the LVEF and level of miR-129 decreased in turn(P<0.05).There was no significant difference in serum miR-129 expression among CSHF patients with coronary heart disease,hypertension heart disease and senile heart valve disease(P>0.05).The serum miR-129 expression of CSHF patients was negatively correlated with LVESD,LVEDD and NYHA grade(P<0.05),and positively correlated with LVEF(P<0.05).Conclusion miR-129 is low expressed in the serum of patients with CSHF.It is negatively related to the LVESD,LVEDD and NYHA grade,and positively related to LVEF,which may provide reference for the evaluation of CSHF.