柴胡疏肝散干预急性心肌梗死后抑郁大鼠海马炎症因子的表达

Observation of Chaihu Shugan decoction intervening the expression of inflammatory factors in hippocampus of rats with depression after acute myocardial infarction

目的探讨柴胡疏肝散干预急性心肌梗死后抑郁大鼠的作用及机制。方法120只SD大鼠,其中90只造模成急性心肌梗死后抑郁后随机分为模型组30只、中药组30只和氟西汀组30只,未造模30只为正常组,治疗7天、14天、21天后,对各组大鼠进行行为学指标测定,之后处死并分离海马组织,采用酶联免疫吸附法分析白细胞介素1(IL-1)、白细胞介素6(IL-6)、半胱氨酸天冬氨酸蛋白酶9(Caspase-9)和半胱氨酸天冬氨酸蛋白酶3(Caspase-3),采用蛋白免疫印迹法及反转录聚合酶链反应检测氨基末端激酶3(JNK3)的表达水平。结果与正常组同期相比较,模型组行为学指标均降低(P<0.05),IL-1、IL-6、Caspase-3、Caspase-9、JNK3显著升高(P<0.05),而中药组和氟西汀组敞箱试验得分减少(P<0.05),在7天、14天后氟西汀组、中药组炎症因子均明显增高(P<0.05),而21天后中药组除IL-1、JNK3和Caspase-9明显增高而氟西汀组除IL-1、JNK3明显增高(P<0.05)外余差异无显著性(P>0.05);与模型组同期相比较,中药组、氟西汀组行为学指标明显改善(P<0.05),IL-1、IL-6、Caspase-3、Caspase-9、JNK3显著降低(P<0.05);与氟西汀组同期相比较,21天后中药组IL-1降低(P<0.05)。结论柴胡疏肝散有效改善心肌梗死大鼠抑郁情况,可能是通过抑制大鼠海马炎症来完成的。

Aim To observe the clinical efficacy and mechanism of Chaihu Shugan decoction in rats with depression after acute myocardial infarction.Methods 90 models among 120 SD rats were randomly divided into model group(n=30),Chinese medicine group(n=30)and fluoxetine group(n=30),unmodeled rats were normal group(n=30),after 7 days,14 days,21 days for treatment,behavioral indicators were measured in each group of rats,then hippocampus was sacrificed and isolated,and interleukin-1(IL-1),interleukin-6(IL-6),cysteinyl aspartate specific proteinase-9(Caspase-9)and cysteinyl aspartate specific proteinase-3(Caspase-3)were analyzed by enzyme-linked immunosorbent assay(ELISA),and the expression levels of Jun N-terminal kinase 3(JNK3)were detected by Western blot and reverse transcription-polymerase chain reaction(RT-PCR).Results Compared with normal group in the same period,the behavior indicators of the model group were reduced(P<0.05),IL-1,IL-6,Caspase-3,Caspase-9,and JNK3 were significantly increased(P<0.05),while the open-field test scores of Chinese medicine group and fluoxetine group decreased(P<0.05),and the inflammatory factors were significantly increased in Chinese medicine group and fluoxetine group after 7 days and 14 days(P<0.05).After 21 days,except IL-1,JNK3,Caspase-9 in Chinese medicine group and IL-1,JNK3 in fluoxetine group,there was no significant difference in the remaining indicators compared with normal group(P>0.05).Compared with model group in the same period,the behavior indicators were significantly improved in Chinese medicine group and fluoxetine group(P<0.05),and IL-1,IL-6,Caspase-3,Caspase-9,and JNK3 were significantly reduced(P<0.05).Compared with fluoxetine group in the same period,IL-1 decreased in Chinese medicine group after 21 days(P<0.05).Conclusion Chaihu Shugan decoction can effectively improve the depression of myocardial infarction rats,which may be accomplished by inhibiting the inflammation of hippocampus in rats.