正交试验法优化菱角多糖提取工艺

Orthogonal Test Method to Optimize Extraction Technology of Water Chestnut Polysaccharide

菱角多糖具多种药理活性。为了优选出热水浸提法提取菱角多糖的最佳工艺条件,本研究以菱角多糖的提取率为指标,选取料液比、提取温度、提取时间为影响因素,在综合单因素实验的基础上,采用L9(34)正交试验进行工艺优化,采用苯酚—硫酸法测定多糖的含量。结果表明:多糖提取率随料液比的增加,呈先升后降的趋势,料液比为1:30时提取率最高,为2.72%;提取温度为70℃时,多糖提取率最高,为2.96%;提取时间为120 min时,提取率最高,为1.81%,之后提取率减少。综合以上单因素试验结果进行正交试验,得到菱角多糖的最佳提取工艺为料液比1:30、温度60℃、时间120 min。热水浸提菱角多糖工艺操作简便易行,具有实用价值,可依据该工艺条件制定菱角多糖提取工艺。

Water chestnut polysaccharide has a variety of pharmacological activities.In order to optimize the best process condition for extracting Trapa bispinosa polysaccharide by hot water extraction,in this study,the extraction rate of Trapa bispinosa polysaccharides is taken as an index,and the material-liquid ratio,extraction temperature and extraction time are selected as influencing factors.Based on the comprehensive single-factor experiment,the L9(34)orthogonal experiment is used to optimize the process,and the phenol-sulfuric acid method is used to determine the polysaccharide content.The results show that the extraction rate of polysaccharide increases first and then decreases with the increase of the ratio of material to liquid.When the ratio of material to liquid is 1:30,the extraction rate is the highest(2.72%).When the extraction temperature is 70℃,the extraction rate of polysaccharide is the highest(2.96%).When the extraction time is 120 minutes,the extraction rate is the highest(1.81%),and then the extraction rate decreased.Based on the above single factor test results,an orthogonal test is carried out,and the best extraction process for Trapa bispinosa polysaccharide is 1:30,the extraction temperature is 60℃,and the extraction time is 120 min.The extraction process of Trapa bispinosa polysaccharide by hot water extraction is simple and practical,and the process of extracting Trapa bispinosa polysaccharide can be formulated according to the process conditions.