HPLC测定真菌及其制品中麦角甾醇含量

Determination of Ergosterol in Fungi and Its Products by HPLC

本研究旨在找出真菌及其制品的特征指标,用于其质量评价、掺伪鉴别等。建立了真菌及其制品中麦角甾醇含量测定的高效液相色谱法(HPLC),并利用建立的HPLC法对冬虫夏草、破壁灵芝孢子粉等真菌及其制品以及螺旋藻、大豆油等6种植物油样品进行麦角甾醇含量的测定。结果显示,麦角甾醇在5~160μg/mL范围内具有较好的线性,线性相关系数大于0.999,检出限为0.1μg/mL,平均回收率为86.32%~105.24%。螺旋藻以及大豆油等6种植物油中均未检出麦角甾醇,真菌类及其制品中均含有麦角甾醇,麦角甾醇具有专属性。该方法操作简便、灵敏,且具有较好的重复性和稳定性,可应用于真菌及其制品中麦角甾醇含量的定量分析。

The aim is to find out the characteristic indexes of fungi and its products for quality evaluation and adulteration identification.The HPLC method was established for determining ergosterol in fungi and its products.With the established HPLC method,the contents of ergosterol were determined in fungi and its products(Cordyceps sinensis and Ganoderma lucidum spores powder),spirulina and six kinds of plant oil,such as soybean oil.The results showed that there was a good linearity in the range of 5-160μg/mL of ergosterol,the correlation coefficient was more than 0.999,the detection limit was 0.1μg/mL,and the average recovery was from 86.32%to 105.24%.Ergosterol was not determined in spirulina and six kinds of plant oil(such as soybean oil),while it was detected in fungi and its products.Ergosterol had specificity in fungi and its products.This study suggests that the method is simple and sensitive,and has good repeatability and stability,thus it can be adopted to quantitatively analyze ergosterol in fungi and its products.