重症婴幼儿龋患者口腔念珠菌与菌群的关系

Relationship between oral Candida albicans and flora in children with severe early childhood caries

目的:探讨重症婴幼儿龋患儿口腔念珠菌与菌群的关系。方法 :选择2018年3月-2019年6月北京大学深圳医院治疗的重症婴幼儿龋患儿42例作为实验组,另选择来院进行口腔检查的无龋儿童40例作为对照组。分别采集各组患儿唾液、菌斑标本,进行微生物培养,将实验组组内分为白色念珠菌阴性、阳性患儿。通过高通量lllumina测序平台,对实验组及对照组儿童唾液、牙菌斑整体进行微生物测序,分析细菌多样性及丰度。采用SPSS 23.0软件包对数据进行统计学分析。结果:实验组唾液与菌斑中白色念珠菌阳性率均显著高于对照组(P<0.05)。实验组白色念珠菌阳性患儿唾液中纤毛菌属、心杆菌属丰度显著高于本组阴性患儿(P<0.05),实验组白色念珠菌阳性患儿菌斑中普雷沃菌属丰度显著高于本组阴性患儿及对照组(P<0.05);实验组白色念珠菌阳性患儿唾液中纤毛菌丰度显著高于阴性患儿(P<0.05),牙龈二氧化碳噬纤维菌、人心杆菌、颗粒二氧化碳噬纤维菌丰度显著低于阴性患儿(P<0.05);实验组白色念珠菌阳性患儿菌斑中变形链球菌、格氏乳杆菌、阴道乳酸杆菌丰度显著高于阴性患儿(P<0.05),植物乳杆菌丰度显著低于阴性患儿(P<0.05)。结论:重症婴幼儿龋患儿口腔中存在白色念珠菌,与纤毛菌、牙龈二氧化碳噬纤维菌、人心杆菌、颗粒二氧化碳噬纤维菌、变形链球菌及部分乳杆菌丰度有关,其间可能有相互协同或拮抗作用。

PURPOSE: To investigate the relationship between oral Candida albicans and flora in children with severe infant caries. METHODS: Forty-two children with severe infant caries(experimental group) and 40 caries-free children(control group) treated in Peking University Shenzhen Hospital from March 2018 to June 2019 were enrolled. The samples of saliva and plaque were collected and cultured. According to the culture results, the experimental group was further divided into two subgroups: Candida albicans positive group and Candida albicans negative group. The samples were sequenced by high-throughput sequencing technology, then the diversity and abundance of bacteria were analyzed. The data were processed by SPSS 23.0 software package. RESULTS: The positive rates of Candida albicans in saliva and plaque were significantly higher in the experimental group than in the control group(P<0.05). The abundance of Leptotrichia and Cardiobacterium in the saliva of children with positive Candida albicans in the experimental group was significantly higher than that of children with negative Candida albicans(P<0.05). The abundance of Prevotella in plaque of children with positive Candida albicans in the experimental group was significantly higher than that of children with negative Candida albicans and control group(P<0.05). The abundance of Leptotrichia in saliva of children with positive Candida albicans in the experimental group was significantly higher than that of children with negative Candida albicans(P<0.05), while the abundances of Capnocytophaga, Cardiobacterium hominis, and Capnocytophaga granulose were significantly lower than those of children with negative Candida albicans(P<0.05). The abundance of Streptococcus mutans, Lactobacillus gasseri, and Lactobacillus vaginalis in the plaque of positive Candida albicans in the experimental group were significantly higher than those of children with negative Candida albicans(P<0.05), while the abundance of Lactobacillus plantarum was significantly lower than that of children with negative Candida albicans(P<0.05). CONCLUSIONS: Candida albicans in children with severe infant caries children is closely correlated with the abundance of Leptotrichia, Capnocytophaga, Cardiobacterium hominis, Capnocytophaga granulosa, Streptococcus mutans and Lactobacillus, which may play a synergistic or antagonistic role.