Survivin和VEGF反义寡核苷酸联合转染对舌癌的作用

Suppression of Survivin and VEGF antisense oligonucleotide on growth in tongue squamous cancer cells

目的观察生存素反义寡核苷酸(Survivin-ASODN)与血管内皮细胞生长因子反义寡核苷酸(VEGF-ASODN)单独和联合进行转染对Tca8113舌癌细胞的影响。方法分别构建Survivin-ASODN和VEGF-ASODN,然后进行联合转染Tca8113舌癌细胞,24 h后使用免疫组织化学方法检测癌细胞中Survivin和VEGF蛋白表达情况,癌细胞的凋亡情况采用流式细胞仪进行检测,转染对于细胞的影响采取四唑盐(MTT)比色试验方法检测,同时绘制细胞生长曲线显示转染对于细胞的作用。实验结果数据用SPSS22.0统计学软件进行差别检验。结果Survivin-ASODN和VEGF-ASODN联合转染显著降低了癌细胞Survivin及VEGF蛋白表达(P<0.01),抑制了癌细胞生长及其活性,增加了癌细胞凋亡(P<0.01)。结论Survivin-ASODN和VEGF-ASODN联合转染Tca8113舌癌细胞可以显著抑制癌细胞Survivin和VEGF的蛋白表达水平,增强癌细胞的凋亡,减低癌细胞的增殖。

Objective:To observe the effects of Survivin and vascular endothelial growth factor antisense oligonucleotide(Survivin-ASODN and VEGF-ASODN)on growth in tongue cancer cells.Methods:The Survivin-ASODN and VEGF-ASODN was synthesized artificially with phosphorothioic acid.Survivin-ASODN and VEGF-ASODN were transfecting in tongue cancer cells Tca8113.The quantity of Survivin and VEGF protein was detected by immunohistochemistry.FCM and MTT method were used to detect cellular apoptosis and the activity of cells,respectively.The effect of transfection on the growth of cells was evaluated by Growth curve.SPSS22.0 statistical software was used for statistical analysis.Results:The protein levels of Survivin and VEGF in the cells decreased when Survivin-ASODN and VEGF-ASODN were transfected(P<0.01).The ratio of apoptosis increased,and the activity of cells also decreased as transfected Survivin-ASODN and VEGF-ASODN(P<0.01).Conclusion:Transfection with Survivin-ASODN and VEGF-ASODN in tongue cancer cells Tca8113 can inhibit the expressions of Survivin and VEGF remarkably,which can enhance cellular apoptosis and suppress growth of cells.