IQGAP1在2型糖尿病肾病患者足细胞凋亡中的作用探究

The role of IQGAP1 in podocyte apoptosis in type 2 diabetic nephropathy patients

目的探讨IQ结构域GTP酶活化蛋白1(IQGAP1)在2型糖尿病肾病患者足细胞凋亡中的作用及机制探究。方法采用免疫组织化学法完成两组肾脏组织中IQGAP1、p-ERK1/2、ERK1/2表达水平;采用SPSSPearson相关性分析软件分析IQGAP1与p-ERK1/2、ERK1/2表达及分布关系。结果实验组糖尿病肾病组织中IQGAP1阳性率低于对照组(P<0.05);实验组糖尿病肾病组织中p-ERK1/2、ERK1/2阳性率均高于对照组(P<0.05);免疫组化结果表明:实验组糖尿病肾病组织中足细胞裂隙变窄,足突缩小及伴有节段性融合,可见足细胞凋亡小体形成,IQGAP1分布在肾小球足细胞、系膜细胞、血管内皮细胞中;对照组肾脏组织中未见肾脏病变改变,IQGAP1表达水平较高;SPSSPearson相关性分析结果表明:实验组肾脏组织中IQGAP1与p-ERK1/2、ERK1/2蛋白阳性率呈正相关性(P<0.05)。结论IQGAP1在2型糖尿病肾病患者中呈低表达,且表达水平与p-ERK1/2、ERK1/2存在相关性,可能由于p-ERK1/2、ERK1/2能引起足细胞凋亡,从而引起大量蛋白尿,导致糖尿病肾病的发生。

Objective:To investigate the role and mechanism of IQ domain GTPase activating protein 1(IQGAP1)in podocyte apoptosis in type 2 diabetic nephropathy patients.Methods:The expression levels of IQGAP1,p-ERK1/2 and ERK1/2 in the two groups were determined by SPSSPearson correlation analysis software.The expression and distribution of IQGAP1 and p-ERK1/2 and ERK1/2 were analyzed by SPSSPearson correlation analysis software.Results:The positive rate of IQGAP1 in the experimental group was lower than that in the control group(P<0.05).The positive rate of p-ERK1/2 and ERK1/2 in the diabetic nephropathy group was higher than that in the control group(P<0.05).The results of immunohistochemistry showed that the podocyte fissures in the diabetic nephropathy group in the experimental group were narrowed,the constriction was narrowed and the segmental fusion was accompanied,and the apoptotic bodies of podocytes were formed.The IQGAP1 was distributed in the glomerular podocytes and mesangium.In the cells and vascular endothelial cells,no renal lesions were observed in the kidney tissues of the control group,and the expression level of IQGAP1 was higher.The results of SPSSPearson correlation analysis showed that the positive rate of IQGAP1,p-ERK1/2 and ERK1/2 protein in the kidney tissue of the experimental group Positive correlation(P<0.05).Conclusion:IQGAP1 is down-regulated in patients with diabetic nephropathy,and the expression level is correlated with p-ERK1/2 and ERK1/2.It may be caused by p-ERK1/2 and ERK1/2,which may cause apoptosis of podocytes.Proteinuria,leads to the development of diabetic nephropathy,though further research and discussion are required.