hsa-miR-125a的靶基因预测及生物信息学分析

Target Gene Prediction and Bioinformatics Analysis of hsa-miR-125a

目的:通过生物信息学分析方法预测hsa-miR-125a的靶基因并对靶基因进行GO、KEGG富集分析。方法:利用miRBase数据库分析hsa-miR-125a的物种保守性;利用TargetScan、miRTarBase、miRDB数据库预测hsa-miR-125a靶基因,再将预测的靶基因利用DAVID数据库进行基因本体(GO)功能富集分析及京都基因与基因组百科全书(KEGG)生物通路富集分析。结果:hsa-miR-125a预测的交集靶基因共有122个。GO富集分析结果显示,hsa-miR-125a的靶基因主要参与细胞对DNA损伤刺激的反应、蛋白质泛素化的正调控等生物过程(均P<0.01)。KEGG信号通路富集分析结果显示,hsa-miR-125a的靶基因显著富集于endocytosis signaling pathway、adherens junction signaling pathway、TGF-βsignaling pathway、HIF-1 signaling pathway(P<0.05)。结论:hsa-miR-125a的靶基因富集的信号通路与破骨细胞生成密切相关,尤其是TGF-βsignaling pathway。

Objective:To predict the target genes of hsa-miR-125a by bioinformatics analysis and to analyze the enrichment of GO and KEGG.Methods:miRBase database was used to analyze the species conservatism of hsa-miR-125a;TargetScan,miRTarBase and miRDB databases were used for target gene prediction,and DAVID database was used to analyze gene ontology(GO)function enrichment and Kyoto Encyclopedia of genes and genomes(KEGG)bioaccumulation analysis.Results:There were 122 target genes predicted by hsa-miR-125a.Go analysis showed that the target gene of hsa-miR-125a was mainly involved in cell response to DNA damage and the positive regulation of protein ubiquitination(all P<0.01).KEGG signaling pathway analysis showed that the target genes of hsa-miR-125a were significantly enriched in endocytosis signaling pathway,adhesions junction signaling pathway,TGF-βsignaling pathway and HIF-1 signaling pathway(P<0.05).Conclusion:The signal pathway of hsa-miR-125a target gene enrichment is closely related to osteoclast formation,especially TGF-βsignaling pathway.