乌鲁木齐地区汉族支气管哮喘儿童ADAM33基因S2位点与TGF-β1、ERK水平相关性分析

Correlation analysis of ADAM33 gene S2 locus and TGF-β1,ERK levels in children with bronchial asthma in Urumqi area

目的探讨乌鲁木齐支气管哮喘汉族儿童解整合素-金属蛋白酶33(ADAM33)基因S2、转化生长因子-β1(TGF-β1)、胞外信号调控激酶(ERK)水平的相关性,期望从表观遗传学的水平为哮喘的防治提供新的思路与靶点。方法选择2018年7月—2019年7月新疆医科大学第五附属医院及第一附属医院门诊及入院,3~14岁的汉族支气管哮喘患儿78例作为哮喘组,选择预防保健科就诊的同期45例汉族非哮喘儿童作为非哮喘组。对ADAM33 S2采用聚合酶链反应进行分型,IgE、TGF-β1及ERK水平用酶联免疫吸附分析法检测,比较基因型及等位基因频率在哮喘组与非哮喘组中的差异,进行三者相关性分析。结果两组ADAM33 S2基因型及等位基因频率比较,差异有统计学意义(P<0.05)。非哮喘组IgE、TGF-β1、ERK水平低于哮喘组,差异有统计学意义(P<0.05或P<0.01)。TGF-β1与ADAM33 S2、ERK呈正相关(r>0,P<0.05),ADAM33S基因S2与ERK无相关性(P>0.05)。结论乌鲁木齐汉族哮喘儿童易感基因之一可能是ADAM33基因S2。哮喘组IgE、TGF-β1及ERK指标均高于非哮喘组。TGF-β1可能上调汉族哮喘儿童ADAM33基因S2表达。ERK可能会上调TGF-β1的分泌。

Objective To explore the correlation between disintegrin-metalloproteinase 33(ADAM33)gene S2,transforming growth factor-β1(TGF-β1),and extracellular signal-regulated kinase(ERK)levels in Han nationality children with bronchial asthma in Urumqi.The level provides new ideas and targets for the prevention and treatment of asthma.Methods Outpatients and admissions of the Fifth Affiliated Hospital and First Affiliated Hospital of Xinjiang Medical University from July 2018 to July 2019 were selected,a total of 78 children with bronchial asthma in the Han nationality aged 3 to 14 were selected as the asthma group,and the same period when the preventive health care department was selected 45 cases of non-asthmatic children of Han nationality were regarded as non-asthmatic group.The ADAM33 S2 was typed by polymerase chain reaction,and the levels of IgE,TGF-β1 and ERK were detected by enzyme-linked immunosorbent assay.The differences of genotype and allele frequency between asthma group and non-asthma group were compared,and their correlation were analyzed.Results Comparison of ADAM33 S2 genotype and allele frequency between the two groups was statistically significant(P<0.05).The levels of IgE,TGF-β1,and ERK in the non-asthmatic group were lower than those in the asthma group,and the differences were statistically significant(P<0.05 or P<0.01).TGF-β1 was positively correlated with ADAM33 S2 and ERK(r>0,P<0.05),and ADAM33S gene S2 was not correlated with ERK(P>0.05).Conclusion One of the susceptibility genes in Han nationality children with asthma in Urumqi may be ADAM33 gene S2.The indicators of IgE,TGF-β1 and ERK in the asthma group are higher than those in the non-asthma group.TGF-β1 may up-regulate the expression of ADAM33 gene S2 in Han nationality children with asthma.ERK may up-regulate the secretion of TGF-β1.