果蝇残翅/长翅杂交与PCR结合验证遗传学基本规律

Molecular Marker and Hybridization Combine to Verify the Basic Laws of Genetics in Fruit Fly

为减轻传统有性杂交验证遗传基本规律的工作量以及把传统杂交试验与分子遗传学相结合,利用分子标记和性状共同验证分离及连锁遗传规律,从http://flybase.org/下载果蝇(Drosophila melanogaster)Chr.2R DNA序列并设计引物,筛选长翅和残翅(Vg/vg)果蝇之间多态性连锁PCR标记,获得了2个与Vg/vg紧密连锁的侧翼PCR标记R15和R24,对长翅和残翅杂交后自交得到的F2代个体进行翅型观察统计的同时进行PCR分析。结果表明:自交F_(2)代中Vg/vg、R15/r15分离符合3∶1的分离比,R24/r24的分离符合1∶2∶1的分离比;Vg/vg与R15/r15的交换值为7.24%~8.42%,Vg/vg与R24/r24的交换值为22.82%~23.8%。

To simplify the workload of traditional sexual hybridization to verify the three basic laws of genetics and combine traditional hybridization experiments with molecular genetics,molecular markers and characteristics are used to jointly verify the laws.The DNA sequence of Drosophila melanogaster Chr.2R was downloaded from http://fly⁃base.org/and the PCR primers were designed to screen the polymorphism between long and residual wing(Vg/vg)fruit flies and 2 flank linkage PCR markers R15 and R24 with Vg/vg were obtained.The PCR analysis was carried out at the same time as the wing observation statistics of F_(2) generation individuals obtained by self-interbreeding af⁃ter long-wing and residual wing hybridization.The segregation of Vg/vg、R15/r15 was according with 3∶1,and R24/r24 was according with 1∶2∶1 in F2 population.The cross-over value between Vg/vg and R15/r15 is 7.24%~8.42%,Vg/vg and R24/r24 is 22.82%~23.8%。