Ki67、Ck19与肝细胞癌临床病理特征和磁共振成像表观扩散系数的相关性分析

Correlation analysis of Ki67,Ck19 with clinicopathological features and apparent diffusion coefficient value of hepatocellular carcinoma

目的探讨细胞增殖核抗原(Ki67)、细胞角蛋白19(Ck19)在肝细胞癌(HCC)中的表达,研究其与HCC临床病理特征和磁共振成像表观扩散系数(ADC)值的相关性。方法回顾性收集2014年1月至2019年10月在郑州大学附属肿瘤医院就诊的203例HCC初诊患者的资料,分析所有患者癌组织中Ki67及Ck19的表达和ADC值,以及Ki67和Ck19与患者临床病理特征的关系,并计算与ADC值的差异及相关性。结果Ki67表达阳性和阴性组间的HCC脉管癌栓、甲胎蛋白(AFP)、肿瘤分化程度、复发、淋巴结转移差异有统计学意义(χ^(2)=5.156、12.741、29.925、3.963、77.408,均P<0.05),与ADC值呈负相关(r=-0.214,P=0.002),低Ki67组患者HCC肿瘤的平均ADC值明显高于高Ki67组(1089±280比1009±212,P<0.05);Ck19表达阳性、阴性组间脉管癌栓、AFP、复发、淋巴结转移差异有统计学意义(χ^(2)=9.058、27.034、4.053、14.187,均P<0.05),与ADC值无相关性(r=0.062,P=0.380)。Ki67、Ck19表达在HCC复发时间不同分组之间的差异有统计学意义(均P<0.05)。结论Ki67及Ck19在HCC中的异常表达与HCC术后预后、复发有相关性。Ck19与ADC值之间无相关性,Ki67与ADC值呈负相关,提示ADC值对于术前HCC生物学特性能够提供一定的影像学依据。

Objective To investigate the expression of cell proliferating nuclear antigen(Ki67)and cytokeratin 19(Ck19)in hepatocellular carcinoma(HCC),and correlation with the clinicopathological features and apparent diffusion coefficient(ADC)value of hepatocellular carcinoma.Methods The data of 203 newly diagnosed HCC patients in the Affiliated Cancer Hospital of Zhengzhou University from January 2014 to October 2019 were retrospectively collected,and the expression of Ki67 and Ck19 and ADC values in all patients were analyzed.The relationship between Ki67 and Ck19 and the clinical pathological characteristics of the patient,and calculate the difference and correlation with the ADC value was analyzed.Results There were statistically significant differences in tumor vascular tumor thrombus,alpha-fetoprotein(AFP),postoperative tumor recurrence,lymph node metastasis,and HCC differentiation between Ki67 positive and negative groups(χ^(2)=5.156,12.741,29.925,3.963,77.408,all P<0.05),and negatively correlated with ADC value(r=-0.214,P=0.002).The average ADC value of HCC in the low Ki67 group was significantly higher than that of the high Ki67 group(1089±280 vs 1009±212,P<0.05).There were statistically significant differences in tumor vascular tumor thrombus,AFP,postoperative tumor recurrence,and lymph node metastasis between Ck19 positive and negative groups(χ^(2)=9.058,27.034,4.053,14.187,all P<0.05),but not correlated with ADC value(r=0.062,P=0.380).The expression differences of Ki67 and Ck19 in different HCC recurrence time groups were statistically significant(P<0.05).Conclusion The positive expression of Ki67 and Ck19 in HCC was associated with the prognosis and recurrence of HCC.CK19 was not correlated with the ADC value,while Ki67 was negatively correlated with the ADC value,indicating that ADC value could provide certain imaging information for the biological characteristics of preoperative HCC.