基于MALDI-TOF MS质谱峰分析细菌耐药性的研究进展

Research progress in bacterial resistance to antibiotics analyzed based on MALDI-TOF MS mass peak

基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)已成为微生物鉴定领域广泛应用的方法,其快速、准确、高通量等明显优势吸引众多研究探索其他微生物领域适用范围,当前已报道了多个基于MALDI-TOF MS对细菌进行耐药分析的研究,通过对质谱进行简单的视觉分析或利用信息学和统计学对整个质谱进行更复杂的分析来识别耐药相关的特异质谱峰,因此MALDI-TOF MS成为检测抗生素耐药性的潜在工具,本综述主要阐述了利用MALDI-TOF MS检测细菌耐药性的研究进展。

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS)has become a widely used method in the field of microbial identification.Its obvious advantages including rapidity,great accuracy and high throughput attract many researchers to investigate its potential for usage in other microbiological fields.Currently,several studies have reported MALDI-TOF MS-based analysis of bacterial resistance to antibiotics,which can identify the specific heterogeneous spectrum peaks related to drug resistance through simple visual analysis of the spectrum or more complex analysis of the entire spectrum using informatics methods and statistical approaches.Therefore,MALDI-TOF MS has become a potential tool for detecting antibiotic resistance in bacteria.This review mainly summarized the progress in MALDI-TOF MS-based analysis of bacterial resistance to antibiotics.