龟板联合阿伦磷酸钠预防糖皮质激素性骨质疏松症的实验研究

Experimental study on the prevention of glucocorticoid-induced osteoporosis with tortoise plastron combined with alendronate sodium

目的观察龟板联合阿伦磷酸钠对糖皮质激素性骨质疏松症(GIOP)大鼠骨密度、骨微结构及骨生物力学的影响。方法将36只SD雌性大鼠随机分为基础组、对照组、模型组、龟板组、阿伦磷酸钠组、联合组,每组6只。基础组在实验开始称量体重后即处死取材,模型组、龟板组、阿伦磷酸钠组、联合组采用地塞米松注射液进行造模,连续5周。自造模开始,龟板组同时给予龟板溶液3.15 g/(kg·d)灌胃;阿伦磷酸钠组每周第1天给予阿伦磷酸钠水溶液7.35 mg/kg灌胃,其余6 d给予等体积生理盐水灌胃;联合组每周第1天给予阿伦磷酸钠水溶液7.35 mg/kg灌胃,其余6 d给予龟板溶液3.15 g/(kg·d)灌胃;对照组与模型组每天给予等体积生理盐水灌胃。造模结束后采用双能X射线骨密度测量仪检测骨密度,Micro-CT检测骨微结构[骨面积体积比(BS/TV)、相对骨体积(BV/TV)、骨小梁数量(Tb.N)、骨小梁厚度(Tb.Th)、骨小梁间距(Tb.Sp)、骨小梁模式因子(Tb.Pf)、结构模型指数(SMI)]并进行三维重建,压缩试验检测腰椎骨生物力学参数。结果与模型组比较,龟板组、阿伦磷酸钠组及联合组大鼠全身骨矿盐含量、骨面积、骨密度、BS/TV、BV/TV、Tb.N(龟板组除外)、Tb.Th、最大载荷、压缩位移、压缩刚度及能量吸收值均明显升高(P均<0.05),Tb.Sp、Tb.Pf、SMI均明显降低(P均<0.05);与龟板组及阿伦磷酸钠组比较,联合组大鼠全身骨矿盐含量、骨密度均明显高于龟板组(P均<0.05),联合组大鼠全身骨矿盐含量、骨密度、最大载荷均明显升高(P均<0.05),SMI明显降低(P均<0.05)。结论龟板联合阿伦磷酸钠可明显改善GIOP大鼠骨密度、骨微结构及骨生物力学性能,对GIOP有预防性治疗作用,二者联合使用有协同增效作用。

Objective It is to observe the effects of tortoise plastron combined with alendronate sodium on bone mineral density,bone microstructure and bone biomechanics in rats with glucocorticoid-induced osteoporosis(GIOP).Methods Thirty-six SD female rats were randomly divided into basic group,control group,model group,turtle plastron group,alendronate sodium group,and combination group,with 6 rats in each group.The basic group was sacrificed after weighing the weight at the beginning of the experiment,and the model group,tortoise plastron group,alendronate sodium group,and combination group were modeled with dexamethasone injection for 5 consecutive weeks.Since the beginning of the model building,the tortoise plastron group was given 3.15 g/(kg·d)tortoise plastron solution by gavage;the alendronate sodium group was given 7.35 mg/kg alendronate sodium aqueous solution by gavage on the first day of each week,and was given an equal volume of normal saline by gavage in the remaining 6 days;the combination group was given 7.35 mg/kg alendronate sodium solution on the first day of each week,and was given 3.15 g/(kg·d)tortoise plastron solution by gavage in the remaining 6 days;the control group and the model group were given the same volume of normal saline by gavage every day.After modeling,the bone density was detected by a dual-energy X-ray bone density meter,and the bone microstructure[bone surface/bone volume(BS/TV),bone volume/total volume(BV/TV),trabecular bone number(Tb).N),trabecular bone thickness(Tb.Th),trabecular bone spacing(Tb.Sp),bone trabecular pattern factor(Tb.Pf),structural model index(SMI)]was detected by Micro-CT and three-dimensional reconstruction was performed,biomechanical parameters of lumbar vertebrae were detected by compression test detection.Results Compared with the model group,the content of total bone mineral salt,bone area,bone density,BS/TV,BV/TV,Tb.N(except the tortoise plastron group),Tb.Th,maximum load,compression displacement,compression stiffness and energy absorption value were all significantly increased(all P<0.05),Tb.Sp,Tb.Pf,and SMI were all significantly decreased(all P<0.05);compared with the tortoise plastron group and alendronate sodium group,the content of total bone mineral salt and bone mineral density of the rats in the combination group were significantly higher than those in the tortoise plastron group(P<0.05),the content of total bone mineral salt,bone mineral density and maximum load of the rats in the combination group were significantly increased(all P<0.05),SMI was significantly reduced(all P<0.05).Conclusion The combination of tortoise plastron combined with alendronate sodium can significantly improve the bone mineral density,bone microstructure and bone biomechanical properties of GIOP rats,and has a preventive effect on GIOP.The combination of the two medicine has a synergistic effect.