碱蓬草总生物碱提取工艺优化及其对高脂饮食小鼠的体内抗氧化活性

Extraction of Total Alkaloids from Suaeda salsa and Its Antioxidant Activity in Mice Fed with High-fat Diet

目的:优化碱蓬草总生物碱的提取工艺,研究碱蓬草总生物碱对高脂饮食小鼠的体内抗氧化性。方法:以碱蓬草总生物碱的提取量作为指标,在单因素实验的基础上,进行正交分析。利用碱蓬草的总生物碱对高脂饮食小鼠进行灌胃,定期监测小鼠的衰老指标。结果:碱蓬草总生物碱的最佳提取工艺:超声时间20 min、超声温度45℃、乙醇浓度85%、料液比1:10,在此条件下,总生物碱的提取量为(0.65±0.02)mg/g。抗氧化活性实验表明,碱蓬草总生物碱的低(2 mg/kg)、中(4 mg/kg)、高剂量(8 mg/kg)组均可以显著降低小鼠肝脏中MDA的含量(P<0.05),同时碱蓬草总生物碱的低、中、高剂量组均可以显著增加肝脏中的CAT和GSHPx(P<0.05)。结论:本实验的优化工艺具有良好的可行性,碱蓬草总生物碱具有抗氧化活性,可作为碱蓬草食品及医药方向的研究基础。

Objective:To optimize the extraction process of total alkaloids from Suaeda salsa L.and study the antioxidant activity of total alkaloids from Suaeda salsa on high-fat diet in mice.Methods:The yield of total alkaloids from Suaeda salsa was used as the index,and the orthogonal analysis was carried out on the basis of single factor experiment.The total alkaloids of Suaeda salsa were gavaged to high-fat diet mice,and the aging indexes of mice were monitored regularly.Results:The optimum extraction conditions were as follows:Ultrasonic time 20 min,ultrasonic temperature 45℃,ethanol concentration 85%,solid-liquid ratio 1:10.Under these conditions,the yield of total alkaloids was(0.65±0.02)mg/g.The antioxidant activity test showed that the low,medium and high dose groups of Suaeda salsa total alkaloids could significantly reduce the content of MDA in the liver of mice(P<0.05),and the low(2 mg/kg),medium(4 mg/kg)and high dose(8 mg/kg)groups of total alkaloids of Suaeda salsa could also significantly increase cat and GSH-Px in liver(P<0.05).Conclusion:The optimized process has good feasibility.Suaeda salsa alkaloids can be used as the basis for the study of the total antioxidant activity of Suaeda salsa.