泡桐花多糖的体内外抗氧化活性

Antioxidant Activity in Vitro and Vivo of Polysaccharide from Paulownia fortunei Flower

以泡桐花为原料水提醇沉法制备多糖,测定泡桐花多糖的体外、体内抗氧化活性。体外抗氧化测定结果显示,泡桐花多糖对超氧阴离子、羟基自由基清除以及抑制H2O2溶血有较好的效果,呈剂量依赖性;质量浓度0.5 mg/mL时,对超氧阴离子和羟基自由基清除率分别达到54.36%,74.62%,超过半数效应50%;质量浓度2 mg/mL时,抗H2O2氧化溶血率超过阳性对照组Vc(1 mg/mL)。体内抗氧化测定结果显示,泡桐花多糖低、中、高(日剂量5、10、20 mg/mL,0.2 mL)小鼠灌胃28 d对体质量无影响;血清和心脏、肝脏、肾脏、回肠脏器超氧化物歧化酶(superoxide dismutase,SOD)、还原型谷胱甘肽(glutathione,GSH)、丙二醛(malondialdehyde,MDA)含量和总抗氧化能力(total antioxidant capacity, T-AOC)检测表明,与空白组相比,不同剂量的泡桐花多糖能够增加或显著增加SOD、GSH含量和T-AOC水平(P<0.05),同时降低MDA含量,并存在一定的量效关系。研究表明泡桐花多糖具有良好的抗氧化活性,可作为天然抗氧化剂应用于食药工业。

This study aimed at evaluating the antioxidant activity in vitro and vivo of polysaccharide prepared from Paulownia fortunei flower by water extract-alcohol precipitation.In vitro,results showed that Paulownia fortunei flower polysaccharide had a dose-dependent and good effect on scavenging superoxide anion,hydroxyl radical and inhibiting H2O2 hemolysis.When the concentration of polysaccharide controlled at 0.5 mg/mL,the scavenging rates of superoxide anion and hydroxyl radical were 54.36%and 74.62%respectively,with more than half effect of 50%.When at 2 mg/mL,the anti-H2O2 oxidation hemolysis rate was higher than that of the positive control Vc group(1 mg/mL).In vivo,after the experimental mice were administered to oral gavage with Paulownia flower polysaccharide at low,medium and high dosages(5,10 and 20 mg/mL,0.2 mL)for 28 consecutive days.The result showed that Paulownia fortunei flower polysaccharide had no effect on weight of mice;different doses of polysaccharide could enhance or siginificantly enhance the content of superoxide dismutase(SOD),glutathione(GSH),total antioxidant capacity(T-AOC)remarkably in serum,heart,liver,kidney,ileum(P<0.05)when compared with that of the blank group,and reduce the malondialdehyde(MDA)content dramatically with a certain concentration-response relationship.The results indicate that Paulownia fortunei flower polysaccharide had perfect antioxidant activity and could be used as a natural antioxidant in food and medicine industry.