鹿茸乙醇提取物对秀丽隐杆线虫抗衰老的作用

Anti-aging Effect of Ethanol Extract of Velvet Antler on Caenorhabditis elegans

以秀丽隐杆线虫为模式生物,探究鹿茸乙醇提取物对秀丽隐杆线虫抗衰老的作用。通过寿命、紫外应激、热应激、运动、产卵等实验检测鹿茸乙醇提取物对线虫衰老的影响;通过检测鹿茸乙醇提取物对野生型线虫和突变体线虫抗氧化应激能力的实验,初步探究其衰老作用及机理。结果表明,鹿茸乙醇提取物为2.5 mg/mL时能延长线虫寿命,最长寿命为28 d;具有抗紫外和热应激的能力,线虫平均寿命分别为2.81和3.28 d,优于对照组;能提高线虫运动和产卵能力;氧化应激条件下能显著(P<0.05)延长野生型线虫生存时间;加药组与对照组的clk-1突变体线虫在氧化应激条件下生存时间无明显差异,表明其作用机理可能为激活线粒体信号通路,上调抗氧化基因gst-4、gst-7和skn-1及热抗性基因hsp16.2的转录水平。因此,鹿茸乙醇提取物对线虫具有抗衰老的作用。

Taking Caenorhabditis elegans as model organism,the anti-aging effect of ethanol extract of velvet antler on Caenorhabditis elegans was studied.The effects of ethanol extract of velvet antler on the aging of C.elegans were tested by lifespan,UV stress,heat stress,exercise and oviposition.The anti-oxidative stress of wild-type nematodes and mutant nematodes were detected by ethanol extract of velvet antler.The results showed that the ethanol extract of velvet antler at 2.5 mg/mL could prolong the lifespan of nematodes,the longest lifespan was 28 days.The ethanol extract of velvet antler could resist UV and heat stress,the average lifespan of nematodes was 2.81 and 3.28 days,respectively,which was better than that of the control group.The ethanol extract of velvet antler could improve the locomotion and oviposition of nematodes.Under oxidative stress,the survival time of wild-type nematode was significantly prolonged(P<0.05).The survival time of clk-1 mutant nematode in the treatment group and the control group was not significantly different under oxidative stress,indicating that the mechanism of action might be activation of mitochondrial signaling pathway and the transcription levels of anti-oxidation genes gst-4,gst-7 and skn-1 and heat resistance hsp16.2 were up-regulated.Therefore,ethanol extract of velvet antler has anti-aging effect on nematode.