PRAME mRNA和SOX9 mRNA在肺腺癌中的表达及临床意义

Expression of PRAME mRNA and SOX9 mRNA in lung adenocarcinoma and its clinical significance

目的探讨黑色素瘤特异性抗原(PRAME)mRNA和性别决定基因盒9(SOX9)mRNA在肺腺癌中的表达及临床意义。方法选取2015年2月至2018年4月于该院接受手术治疗的73例肺腺癌患者为研究对象,术中收集肺腺癌癌组织及癌旁正常组织,采用实时荧光定量PCR检测不同组织中PRAME mRNA、SOX9 mRNA的相对表达水平并进行比较;分析肺腺癌患者癌组织PRAME mRNA、SOX9 mRNA表达与临床病理特征的关系;采用Spearman相关分析PRAME mRNA表达与SOX9 mRNA表达的相关性;分析PRAME mRNA、SOX9 mRNA表达与肺腺癌患者预后的关系;采用COX回归模型分析影响肺腺癌患者预后的因素。结果与癌旁正常组织相比,肺腺癌癌组织PRAME mRNA的相对表达水平降低(P<0.05),SOX9 mRNA的相对表达水平升高(P<0.05)。PRAME mRNA、SOX9 mRNA表达与肺腺癌患者性别、年龄、肿瘤大小无关(P>0.05),与病理分级、临床分期、淋巴结转移有关(P<0.05)。Spearman相关分析显示,肺腺癌癌组织PRAME mRNA表达与SOX9 mRNA表达呈负相关(P<0.05)。与预后良好组相比,预后不良组肺腺癌患者PRAME mRNA的相对表达水平降低(P<0.05),SOX9 mRNA的相对表达水平升高(P<0.05)。COX回归模型分析结果显示,PRAME mRNA低表达、SOX9 mRNA高表达、低分化、临床分期Ⅲ期、有淋巴结转移均是影响肺腺癌患者预后的独立危险因素(P<0.05)。结论肺腺癌癌组织中PRAME mRNA、SOX9 mRNA表达异常,且两者呈负相关,可能为肺腺癌的病情及预后评估提供参考依据。

Objective To investigate the expression and clinical significance of preferentially expressed antigen of melanoma(PRAME)mRNA and sex-determining region Y box 9(SOX9)mRNA in lung adenocarcinoma.Methods Totally 73 patients with lung adenocarcinoma who were treated in the hospital from February 2015 to April 2018 were selected as the study objects.The tissues of lung adenocarcinoma and adjacent normal tissues were collected during the operation.The relative expression levels of PRAME mRNA and SOX9 mRNA in different tissues was detected by real-time quantitative PCR,and the relationship between the expression of PRAME mRNA and SOX9 mRNA in the focus tissues of patients with lung adenocarcinoma and the clinicopathological characteristics was analyzed.Spearman correlation was used to analyze the correlation between the expression of PRAME mRNA and SOX9 mRNA.The relationship between the expression of PRAME mRNA and SOX9 mRNA and the prognosis of patients with lung adenocarcinoma was analyzed.Cox regression model was used to analyze the factors affecting the prognosis of patients with lung adenocarcinoma.Results Compared with adjacent normal tissues,the relative expression level of PRAME mRNA in lung adenocarcinoma tissue was lower(P<0.05),and the relative expression level of SOX9 mRNA was higher(P<0.05).The expression of PRAME mRNA and SOX9 mRNA was not correlated with gender,age and tumor size(P>0.05),but correlated with pathological grade,clinical stage and lymph node metastasis(P<0.05).Spearman correlation analysis showed that the expression of PRAME mRNA was negatively correlated with SOX9 mRNA in lung adenocarcinoma(P<0.05).Compared with good prognosis group,the relative expression level of PRAME mRNA was lower and the relative expression level of SOX9 mRNA was higher in poor prognosis group(P<0.05).COX regression model analysis showed that low expression of PRAME mRNA,high expression of SOX9 mRNA,poor differentiation,stageⅢclinical stage and lymph node metastasis were independent risk factors for the prognosis of patients with lung adenocarcinoma(P<0.05).Conclusion The abnormal expression of PRAME mRNA and SOX9 mRNA may be involved in the occurrence and progression of lung adenocarcinoma,and the two are negatively correlated.It may provide a reference for the condition evaluation and prognosis evaluation of lung adenocarcinoma and targeted therapy.