血清miR-16-5p、miR-17-5p和miR-330-3p联合检测对妊娠期糖尿病的诊断价值

Diagnostic value of combined detection of serum miR-16-5p,miR-17-5p and miR-330-3p in gestational diabetes mellitus

目的探讨血清miR-16-5p、miR-17-5p和miR-330-3p对妊娠期糖尿病的诊断价值。方法选择2018年5—6月该院收治的妊娠期糖尿病患者10例和妊娠24~28周的健康孕妇10例,采用转录组测序技术(RNA-seq)分析血清miR-16-5p、miR-17-5p和miR-330-3p的相对表达水平。选择2018年5月至2020年5月该院收治的妊娠期糖尿病患者100例为观察组,妊娠24~28周的健康孕妇100例为对照组。通过实时荧光定量PCR检测2组受试者血清miR-16-5p、miR-17-5p和miR-330-3p的相对表达水平。采用受试者工作特征曲线(ROC曲线)分析血清miR-16-5p、miR-17-5p和miR-330-3p单独或联合检测对妊娠期糖尿病的诊断价值。结果RNA-seq检测结果显示,10例妊娠期糖尿病患者血清miR-16-5p、miR-17-5p和miR-330-3p的相对表达水平较10例健康孕妇高8倍以上。观察组血清miR-16-5p、miR-17-5p和miR-330-3p的相对表达水平均高于对照组,差异有统计学意义(P<0.05)。血清miR-16-5p、miR-17-5p和miR-330-3p单独检测对妊娠期糖尿病的诊断灵敏度分别为95%、97%、94%,特异度分别为95%、96%、95%,血清miR-16-5p、miR-17-5p和miR-330-3p联合检测对妊娠期糖尿病的诊断灵敏度为98%,特异度为93%,准确性为96%。结论血清miR-16-5p、miR-17-5p和miR-330-3p可作为妊娠期糖尿病的潜在诊断标志物。

Objective To investigate the diagnostic value of serum miR-16-5p,miR-17-5p and miR-330-3p in gestational diabetes mellitus.Methods From May to June 2018,10 patients with gestational diabetes mellitus and 10 healthy pregnant women(24-28 weeks)were selected in the hospital.The relative expression levels of miR-16-5p,miR-17-5p and miR-330-3p in serum was analyzed by RNA-seq.Totally 100 patients with gestational diabetes mellitus from May 2018 to May 2020 were selected as observation group,and 100 healthy pregnant women(24-28 weeks)were selected as control group.The relative expression levels of miR-16-5p,miR-17-5p and miR-330-3p were detected by real-time fluorescent quantitative PCR.Receiver operating characteristic curve(ROC curve)was used to analyze the diagnostic efficacy of single or combined detection of serum miR-16-5p,miR-17-5p and miR-330-3p in gestational diabetes mellitus.Results The results of RNA-seq showed that the relative expression levels of miR-16-5p,miR-17-5p and miR-330-3p in serum of 10 patients with gestational diabetes mellitus were more than 8 times higher than those of 10 healthy pregnant women.The relative expression levels of miR-16-5p,miR-17-5p and miR-330-3p in the observation group were higher than those in the control group(P<0.05).The diagnostic sensitivity of single detection serum miR-16-5p,miR-17-5p and miR-330-3p for gestational diabetes mellitus was 95%,97%and 94%respectively,and the specificity was 95%,96%and 95%respectively.The sensitivity,specificity and accuracy of combined detection of serum miR-16-5p,miR-17-5p and miR-330-3p were 98%,93%and 96%,respectively.Conclusion Serum miR-16-5p,miR-17-5p and miR-330-3p can be used as potential diagnostic markers of gestational diabetes mellitus.