循环肿瘤细胞的分离与鉴定研究进展

Research progress on isolation and identification of circulating tumor cells

循环肿瘤细胞(CTC)是由自发或诊疗操作中从实体瘤、转移灶进入外周血循环系统的肿瘤细胞,是“液体活检”的一项重要指标,可以为肿瘤的早期诊断、转移复发的评估提供有力帮助。但由于体液中CTC的数量极少,检测CTC首先需要将细胞高效分离出来,并对细胞纯度、表型进行鉴定。目前已经建立了较多分离、鉴定CTC的方法,本文就CTC的分离与鉴定方法进行综述,并对其未来的应用进行展望。

Circulating tumor cells(CTC)are tumor cells that enter the peripheral blood circulation system from solid tumors and metastases by spontaneous or diagnostic operation.It is an important index of"liquid biopsy",which can provide powerful help for the early diagnosis of tumors and the evaluation of metastasis and recurrence.However,as CTC is extremely rare in body fluids,it is necessary to isolate the cells efficiently and identify the purity and phenotype of the cells.At present,many methods of CTC isolation and identification have been established.This paper reviews the methods of CTC isolation and identification,and prospects its future application.