HPLC法测定藏药三果汤中没食子基儿茶素没食子酸酯和表没食子基儿茶素没食子酸酯的含量

Determination of EGCG and GCG in Tibetan Medicine Triphala Decoction by HPLC

目的:建立藏药三果汤中没食子基儿茶素没食子酸酯(GCG)和表没食子基儿茶素没食子酸酯(EGCG)的HPLC含量测定方法。方法:Agilent Zorbax C18键合硅胶柱(150 mm×4.6 mm,5μm);乙腈-0.1%磷酸梯度洗脱;流速:1.0 mL·min^(-1);检测波长:280 nm;柱温:25℃;进样量:10μL。结果:EGCG和GCG在浓度0.0231~0.2310 mg·mL^(-1)、0.0084~0.0840 mg·mL^(-1)范围内呈良好的线性关系,相关系数均大于0.9990;加样回收率分别为100.09%和99.52%,RSD值分别为0.95%和1.03%(n=6)。结论:建立HPLC法同时检测藏药三果汤中EGCG和GCG的含量,该方法简便、准确、重复性好,结果可靠,可为藏药三果汤中相关成分的质控提供参考。

Objective A high performance liquid chromatography(HPLC)method was established for the determination of epigallocatechin gallate(EGCG)and gallocatechin gallate(GCG)in Tibetan medicine Triphala Decoction.Methods The chromatographic column is an Agilent Zorbax C18 bonded silica column(150 mm×4.6 mm,5μm),the mobile phase is acetonitrile-0.1%phosphoric acid gradient elution,the flow rate is 1.0 mL·min^(-1),the detection wavelength is 280 nm,the column temperature is 25℃,and the injection volume is 10μL.Results EGCG and GCG showed a good linear relationship in the concentration range of 0.0231~0.2310 mL·min^(-1) and 0.0084~0.0840 mL·min^(-1),and the correlation coefficients were both greater than 0.9990;the sample recovery rates were 100.09%and 99.52%,respectively,and the RSD value They are 0.95%and 1.03%respectively(n=6).Conciusion An HPLC method was established to simultaneously detect the contents of EGCG and GCG in Tibetan medicine Sanguo Decoction.This method is simple,accurate,reproducible,and reliable.It can provide a reference for the quality control of related components in Tibetan medicine Triphala Decoction.