高表达活化白细胞黏附因子对AZ-521胃癌细胞系增殖能力的影响

Effects of overexpression activated leukocyte cell adhesion molecule on cell proliferative activity in AZ-521 cells

目的:探讨活化白细胞黏附分子(Activated leukocyte cell adhesion molecule,ALCAM),即CD166对AZ-521胃癌细胞系增殖能力的影响。方法:以pLOX-CWBmi1-CD166慢病毒质粒感染胃癌细胞株AZ-521,采用荧光定量PCR以及Western blot分别测定CD166 mRNA和蛋白表达情况;采用CCK-8测定细胞增殖情况;采用吉姆萨染色测定细胞克隆形成数。结果:pLOX-CWBmi1-CD166慢病毒质粒感染胃癌细胞株AZ-521后CD166 mRNA和蛋白水平均明显增加(P<0.05);细胞增殖能力明显增加(P<0.05);细胞克隆形成数量明显增多(P<0.05)。体内成瘤实验显示,CD166过表达小鼠肿瘤体积明显大于对照细胞(P<0.05)。结论:CD166可促进AZ-521胃癌细胞系增殖。

Objective:To explore the roles of activated leukocyte cell adhesion molecule(ALCAM,also known as CD166)on cell proliferative activity in AZ-521 cells.Methods:Human gastric adenocarcinoma cells AZ-521 were transfected with pLOX-CWBmi1-CD166 lentiviral plasmid,then the expression of CD166 mRNA and protein were determined by fluorescence quantitative PCR and Western blot,respectively.CCK-8 was used to determine cell proliferation.Giemsa staining was used to determine the number of cell clone formation.Results:Compared with control cells,CD166 mRNA and protein levels of CD166 overexpressing cells significantly increased(P<0.05),and the ability of cell proliferation significantly increased(P<0.05).The number of cell clones significantly increased(P<0.05).The tumor volume of CD166 overexpression mice was significantly larger than that of control mice(P<0.05).Conclusions:High expression of CD166 protein enhances cell proliferation in AZ-521 cells.