甘菊木质素调控转录因子ClSND1-like的克隆与表达分析

Clonging and Expression Analysis of Lignin-regulated Transcription Factor ClSND1-like from Chrysanthemum lavandulifolium

为了探索纤维特异表达的NAC类转录因子SND1(secondary wall-associated NAC domain protein1,SND1)在甘菊(Chrysanthemum lavandulifolium)生长发育及非生物胁迫中的作用,该研究从甘菊中克隆了ClSND1-like基因,并对其进行生物信息学、亚细胞定位及表达模式分析。结果发现:(1)ClSND1-like基因的编码区全长1185 bp,共计编码394个氨基酸,是一个不稳定的亲水性蛋白,与除虫菊基因亲缘关系较近。(2)亚细胞定位结果表明,ClSND1-like基因在叶中不表达,在茎的导管壁上特异表达,属于木质素特异表达基因。(3)实时荧光定量PCR结果发现,ClSND1-like基因在茎中表达量最高,在根和叶中表达量极低,且随着茎秆的老化,表达量逐渐升高;在渗透胁迫和盐胁迫中表达量均显著提高。研究推测,ClSND1-like基因与甘菊木质素形成相关,并参与调控植物生长及盐和渗透胁迫。该结果为ClSND1-like基因在甘菊生长和非生物胁迫抗性中的研究奠定了基础,也为菊属植物的生长及抗性研究提供了新思路。

The purpose of this study was to explore the role of SND1(secondary wall associated NAC domain protein1,SND1),a fiber-specific NAC transcription factor,on the growth and abiotic stress of Chrysanthemum lavandulifolium.In this study,ClSND1-like gene was cloned from C.lavandulifolium and its bioinformatics,subcellular localization and expression pattern analysis were performed.The results showed as follow:(1)the full length of ClSND1-like gene CDS is 1185 bp,encoding 395 amino acids,which are unstable hydrophobic amino acids and more closely related to pyrethrum gene.(2)The results of subcellular localization showed that the ClSND1-like gene was not expressed in leaves,but was specifically expressed in the duct wall of stem,which was a lignin specific gene.(3)Real-time quantitative PCR results showed that the expression level of ClSND1-like gene was the highest in stem,and very low in root and leaf,and the expression level gradually increased with the aging of stem.The expression levels were significantly increased in osmotic stress and salt stress.These results suggest that the ClSND1-like gene may be related to lignin formation,and may be involved in regulating plant growth and salt and osmotic stress.This study lays a foundation for the study of both growth and abiotic stress resistance of ClSND1-like gene in C.lavandulifolium,and also provides a new idea for the study of growth and resistance of Chrysanthemum plants.