沉默calnexin对舌鳞状细胞癌细胞增殖、侵袭和迁移的影响

The effect of silencing the endoplasmic reticulum stressrelated protein calnexin on the proliferation,invasion,and migration of tongue squamous cell carcinoma cells

目的探讨沉默内质网应激相关蛋白calnexin对舌鳞状细胞癌细胞增殖、侵袭和迁移的影响。方法将calnexin siRNA转染到舌鳞状细胞癌细胞SCC9和SCC25中,采用qRTPCR检测calnexin表达情况;Western blot实验进一步验证calnexin siRNA沉默效果。通过CCK8实验检测沉默calnexin对舌鳞状细胞癌细胞增殖的影响;运用Transwell法检测沉默calnexin对细胞侵袭和迁移能力的影响。结果qRTPCR显示calnexin siRNA能够有效沉默calnexin的表达;Western blot实验进一步证实calnexin siRNA对calnexin的沉默效果。CCK8实验显示沉默calnexin表达的第4天,第5天能够抑制舌鳞状细胞癌细胞的增殖,差异有统计学意义(P<0.01);Transwell实验提示沉默calnexin的表达能够抑制舌鳞状细胞癌细胞的侵袭和迁移(P<0.001)。结论沉默calnexin可抑制舌鳞状细胞癌细胞的增殖、侵袭和迁移。

Objective To investigate the effect of silencing the endoplasmic reticulum stressrelated protein calnexin on the proliferation,invasion,and migration of tongue squamous cell carcinoma cells.Methods Calnexin siRNA was transfected into SCC9 and SCC25 tongue squamous cell carcinoma cells,and the expression of calnexin was detected by qRTPCR.The silencing effect of calnexin siRNA was further verified by Western blotting.CCK8 assay was applied to detect the effect of silencing calnexin on the proliferation of tongue squamous cell carcinoma cells;Transwell assay was used to detect the effect of silencing calnexin on the invasion and migration of tongue squamous cell carcinoma cells.Results qRTPCR showed that calnexin siRNA could effectively downregulate the expression of calnexin.Western blot analysis further confirmed the silencing effect of calnexin siRNA on calnexin.The CCK8 assay showed that silencing calnexin expression on the 4th and 5th days could inhibit the proliferation of tongue squamous cell carcinoma cells,and the difference was statistically significant(P<0.01).The Transwell assay showed that knockdown of calnexin could inhibit the invasion and migration of tongue squamous cell carcinoma cells(P<0.001).Conclusion Knockdown of calnexin can inhibit the proliferation,invasion,and migration of tongue squamous cell carcinoma cells.