敲低SIX4对食管癌细胞系增殖能力的影响

Effect of knock-down SIX4 on proliferation ability of esophageal cancer cell

目的研究敲低SIX4对食管癌细胞系增殖能力的影响。方法在食管癌细胞中找到SIX4高表达的细胞系;用慢病毒介导的shRNA敲低技术干扰SIX4的表达,构建出SIX4稳定敲低的细胞系;利用CCK-8试剂盒和平板克隆形成实验检测敲低SIX4后对食管癌细胞系增殖能力的影响。结果食管癌细胞系KYSE70和KYSE450中SIX4的表达量相对较高,在KYSE70和KYSE450中敲低SIX4后显著抑制细胞的增殖能力。结论 SIX4的高表达可以促进食管癌细胞的增殖,在KYSE70和KYSE450中敲低SIX4后显著抑制细胞的增殖能力。

Objective To study the effect of knock-down SIX4 on the proliferation of esophageal cancer cell lines.Methods To find cell lines with high level of SIX4 in esophageal cancer cells;the expression of SIX4 was interfered by lentivirus-mediated shRNA knockdown technique,and a stable knockdown cell line of SIX4 was constructed. CCK-8 kit and plate clone formation assay were used to detect the effect of knockdown SIX4 on the proliferation of esophageal cancer cell lines.Results The levels of SIX4 in KYSE70 and KYSE450 were relatively high,and knock-down of SIX4 significantly inhibited the proliferation of KYSE70 and KYSE450. Conclusion The high expression of SIX4 can promote the proliferation of esophageal cancer cells,and knock-down of SIX4 can significantly inhibit the proliferation of KYSE70 and KYSE450 cells.