消癌止痛散对小鼠镇痛作用及对血清β-内啡肽、前列腺素E2水平的影响

Analgesic Effect of Xiaocan Zhitong Powder on Mice and Its Effect on Serum β-Endorphin and Prostaglandin E Levels

目的探讨消癌止痛散对小鼠的镇痛作用以及对血清β-内啡肽(β-EP)、前列腺素E2(PGE2)水平的影响。方法选取SPF级的昆明小鼠30只,随机分为空白对照组(面粉)、消癌止痛散(0.4 g/只、0.8 g/只)共3组,每组10只并进行温浴缩尾法镇痛实验;另选取SPF的昆明小鼠24只,随机分为空白对照组(面粉)、模型组、消癌止痛散(0.4g/只、0.8g/只)共4组,每组6只,先进行醋酸扭体法镇痛实验,完成醋酸扭体实验后取小鼠血清,通过酶联免疫吸附试验法(ELISA)检测血清PGE2和β-EP水平。结果给药后15、30、45、60、90 min,消癌止痛散低剂量组、高剂量组小鼠的缩尾反应潜伏期(TCL)长于给药前,差异有统计学意义(P<0.05);给药后120min,消癌止痛散低剂量组、高剂量组小鼠的TCL与给药前比较,差异无统计学意义(P>0.05)。醋酸扭体法观察到消癌止痛散组的低剂量组和高剂量组小鼠的扭体次数较模型组明显减少(P<0.05);模型组小鼠与空白对照组比较,血清PGE2水平明显增高(P<0.05),血清β-EP水平明显降低(P<0.05),消癌止痛散组高剂量组与模型组比较,血清PGE2水平明显降低(P<0.01)。结论消癌止痛散可提高疼痛小鼠的TCL和痛阈值,有效减少小鼠体内PGE2水平,增加β-EP水平,具有明显的镇痛作用。

Objective To study the analgesic effect of Xiaocan Zhitong Powder on mice and its effect on serum β-endorphin and prostaglandin E2 levels. Methods A total of 30 qualified Kunming mice were randomly divided into 3 groups: a blank control group(flour) and a Xiaocanzhitong Powder(0.4 g/only, 0.8 g/only). Each group of 10 was tested by the warm bath tail-shrink method;24 qualified Kunming mice were selected and randomly divided into a blank control group(flour), a model group, and Xiaocan Zhitong San(0.4 g/only, 0.8 g/only) in a total of 4 groups, 6 in each group, and acetic acid twisted first The analgesia test was performed in vivo, and the serum was taken from the mice after the acetic acid writhing test, and the contents of PGE2 and β-endorphin in the serum were measured by ELISA. Comparative analysis was performed between the groups. Results The tail shrinkage reaction latency and pain were measured at the time points of 15, 30, 45, 60, and 90 minutes after administration in the Xiaocanzhitongsan group of mice, whether in the low-dose group or the high-dose group. The thresholds were all higher than before administration(P<0.05);at 120 min, the latency and pain thresholds of the tail-reaction response of mice in each group were restored to the levels before administration. In the acetic acid twist method, the number of twists in the low-dose and high-dose groups of the Xiaocanzhitongsan group was significantly reduced compared with the model group(P<0.01). Compared with the blank control group, the mice in the model group had a PGE2 content in the serum. Significantly increased(P<0.01), β-endorphin content in serum was significantly reduced(P<0.05), PGE2 content in serum was significantly reduced in the high-dose group of the Xiaocan Zhitong Powder group compared with the model group(P<0.01). Conclusion Xiaocanzhitong Powder has increased the latency and pain threshold of the tail-reaction response in painful mice, and the content of PGE2 in the mice is reduced, and the content of β-endorphin is increased, which has obvious analgesic effect.